ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity

Elisa Oricchio, Chiara Saladino, Stefano Iacovelli, Silvia Soddu, Enrico Cundari

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

We previously showed that ATM is responsible for p53 phosphorylation at Ser15 and localization at centrosomes during mitosis. When p53 centrosomal localization is prevented by inhibiting polymerization of spindle microtubules, a stabilized form of p53 is transmitted to daughter cells that arrest in the next G 1 phase of the cell cycle after exit from mitosis. AT cells are unable to both localize p53 at centrosomes in mitosis and arrest after exposure to mitotic-spindle poisons. Here we show that during mitosis ATM is activated by phosphorylation at Ser1981 and localizes at centrosomes. When mitotic spindle is disrupted by nocodazole, ATM is displaced from centrosomes and colocalizes with phospho-Ser15-p53 under the form of spots dispersed in the mitotic cytoplasm. After release from nocodazole-block, as soon as cells exit mitosis, p53 is redirected to the nucleus and its Ser15 phosphorylation is substituted by phosphorylation at Ser46. We suggest that ATM is activated by default at each mitotic onset and phosphorylates p53 at Ser15 so as to keep it inactive at centrosomes when the spindle is correctly in place or, in case of inactivation of the mitotic spindle, to maintain the memory of a perturbed mitosis.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalCell Cycle
Volume5
Issue number1
Publication statusPublished - Jan 1 2006

Fingerprint

Centrosome
Phosphorylation
Spindle Apparatus
Automatic teller machines
Mitosis
Nocodazole
Poisons
Polymerization
Cells
Data storage equipment
Microtubules
Cell Cycle
Cytoplasm

Keywords

  • ATM
  • Centrosomes
  • Mitosis
  • p53
  • Phosphorylation
  • Post-mitotic checkpoint

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity. / Oricchio, Elisa; Saladino, Chiara; Iacovelli, Stefano; Soddu, Silvia; Cundari, Enrico.

In: Cell Cycle, Vol. 5, No. 1, 01.01.2006, p. 88-92.

Research output: Contribution to journalArticle

Oricchio, E, Saladino, C, Iacovelli, S, Soddu, S & Cundari, E 2006, 'ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity', Cell Cycle, vol. 5, no. 1, pp. 88-92.
Oricchio, Elisa ; Saladino, Chiara ; Iacovelli, Stefano ; Soddu, Silvia ; Cundari, Enrico. / ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity. In: Cell Cycle. 2006 ; Vol. 5, No. 1. pp. 88-92.
@article{69917ea0573040298e91b2a0d768b261,
title = "ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity",
abstract = "We previously showed that ATM is responsible for p53 phosphorylation at Ser15 and localization at centrosomes during mitosis. When p53 centrosomal localization is prevented by inhibiting polymerization of spindle microtubules, a stabilized form of p53 is transmitted to daughter cells that arrest in the next G 1 phase of the cell cycle after exit from mitosis. AT cells are unable to both localize p53 at centrosomes in mitosis and arrest after exposure to mitotic-spindle poisons. Here we show that during mitosis ATM is activated by phosphorylation at Ser1981 and localizes at centrosomes. When mitotic spindle is disrupted by nocodazole, ATM is displaced from centrosomes and colocalizes with phospho-Ser15-p53 under the form of spots dispersed in the mitotic cytoplasm. After release from nocodazole-block, as soon as cells exit mitosis, p53 is redirected to the nucleus and its Ser15 phosphorylation is substituted by phosphorylation at Ser46. We suggest that ATM is activated by default at each mitotic onset and phosphorylates p53 at Ser15 so as to keep it inactive at centrosomes when the spindle is correctly in place or, in case of inactivation of the mitotic spindle, to maintain the memory of a perturbed mitosis.",
keywords = "ATM, Centrosomes, Mitosis, p53, Phosphorylation, Post-mitotic checkpoint",
author = "Elisa Oricchio and Chiara Saladino and Stefano Iacovelli and Silvia Soddu and Enrico Cundari",
year = "2006",
month = "1",
day = "1",
language = "English",
volume = "5",
pages = "88--92",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Landes Bioscience",
number = "1",

}

TY - JOUR

T1 - ATM is activated by default in mitosis, localizes at centrosomes and monitors mitotic spindle integrity

AU - Oricchio, Elisa

AU - Saladino, Chiara

AU - Iacovelli, Stefano

AU - Soddu, Silvia

AU - Cundari, Enrico

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We previously showed that ATM is responsible for p53 phosphorylation at Ser15 and localization at centrosomes during mitosis. When p53 centrosomal localization is prevented by inhibiting polymerization of spindle microtubules, a stabilized form of p53 is transmitted to daughter cells that arrest in the next G 1 phase of the cell cycle after exit from mitosis. AT cells are unable to both localize p53 at centrosomes in mitosis and arrest after exposure to mitotic-spindle poisons. Here we show that during mitosis ATM is activated by phosphorylation at Ser1981 and localizes at centrosomes. When mitotic spindle is disrupted by nocodazole, ATM is displaced from centrosomes and colocalizes with phospho-Ser15-p53 under the form of spots dispersed in the mitotic cytoplasm. After release from nocodazole-block, as soon as cells exit mitosis, p53 is redirected to the nucleus and its Ser15 phosphorylation is substituted by phosphorylation at Ser46. We suggest that ATM is activated by default at each mitotic onset and phosphorylates p53 at Ser15 so as to keep it inactive at centrosomes when the spindle is correctly in place or, in case of inactivation of the mitotic spindle, to maintain the memory of a perturbed mitosis.

AB - We previously showed that ATM is responsible for p53 phosphorylation at Ser15 and localization at centrosomes during mitosis. When p53 centrosomal localization is prevented by inhibiting polymerization of spindle microtubules, a stabilized form of p53 is transmitted to daughter cells that arrest in the next G 1 phase of the cell cycle after exit from mitosis. AT cells are unable to both localize p53 at centrosomes in mitosis and arrest after exposure to mitotic-spindle poisons. Here we show that during mitosis ATM is activated by phosphorylation at Ser1981 and localizes at centrosomes. When mitotic spindle is disrupted by nocodazole, ATM is displaced from centrosomes and colocalizes with phospho-Ser15-p53 under the form of spots dispersed in the mitotic cytoplasm. After release from nocodazole-block, as soon as cells exit mitosis, p53 is redirected to the nucleus and its Ser15 phosphorylation is substituted by phosphorylation at Ser46. We suggest that ATM is activated by default at each mitotic onset and phosphorylates p53 at Ser15 so as to keep it inactive at centrosomes when the spindle is correctly in place or, in case of inactivation of the mitotic spindle, to maintain the memory of a perturbed mitosis.

KW - ATM

KW - Centrosomes

KW - Mitosis

KW - p53

KW - Phosphorylation

KW - Post-mitotic checkpoint

UR - http://www.scopus.com/inward/record.url?scp=33645324468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645324468&partnerID=8YFLogxK

M3 - Article

C2 - 16319535

AN - SCOPUS:33645324468

VL - 5

SP - 88

EP - 92

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 1

ER -