ATP modifies the proteome of extracellular vesicles released by microglia and influences their action on astrocytes

Francesco Drago, Marta Lombardi, Ilaria Prada, Martina Gabrielli, Pooja Joshi, Dan Cojoc, Julien Franck, Isabelle Fournier, Jacopo Vizioli, Claudia Verderio

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Extracellular ATP is among molecules promoting microglia activation and inducing the release of extracellular vesicles (EVs), which are potent mediators of intercellular communication between microglia and the microenvironment. We previously showed that EVs produced under ATP stimulation (ATP-EVs) propagate a robust inflammatory reaction among astrocytes and microglia in vitro and in mice with subclinical neuroinflammation (Verderio et al., 2012). However, the proteome of EVs released upon ATP stimulation has not yet been elucidated. In this study we applied a label free proteomic approach to characterize the proteome of EVs released constitutively and during microglia activation with ATP. We show that ATP drives sorting in EVs of a set of proteins implicated in cell adhesion/extracellular matrix organization, autophagy-lysosomal pathway and cellular metabolism, that may influence the response of recipient astrocytes to EVs. These data provide new clues to molecular mechanisms involved in microglia response to ATP and in microglia signaling to the environment via EVs.

Original languageEnglish
Article number910
JournalFrontiers in Pharmacology
Volume8
Issue numberDEC
DOIs
Publication statusPublished - Dec 13 2017

Fingerprint

Microglia
Proteome
Astrocytes
Adenosine Triphosphate
Extracellular Vesicles
Autophagy
Cell Adhesion
Proteomics
Extracellular Matrix

Keywords

  • Astrocyte activation
  • ATP
  • Extracellular vesicles
  • Microglia
  • Proteomics

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

ATP modifies the proteome of extracellular vesicles released by microglia and influences their action on astrocytes. / Drago, Francesco; Lombardi, Marta; Prada, Ilaria; Gabrielli, Martina; Joshi, Pooja; Cojoc, Dan; Franck, Julien; Fournier, Isabelle; Vizioli, Jacopo; Verderio, Claudia.

In: Frontiers in Pharmacology, Vol. 8, No. DEC, 910, 13.12.2017.

Research output: Contribution to journalArticle

Drago, Francesco ; Lombardi, Marta ; Prada, Ilaria ; Gabrielli, Martina ; Joshi, Pooja ; Cojoc, Dan ; Franck, Julien ; Fournier, Isabelle ; Vizioli, Jacopo ; Verderio, Claudia. / ATP modifies the proteome of extracellular vesicles released by microglia and influences their action on astrocytes. In: Frontiers in Pharmacology. 2017 ; Vol. 8, No. DEC.
@article{b1a6bd6131524865ba336f3778aefe6a,
title = "ATP modifies the proteome of extracellular vesicles released by microglia and influences their action on astrocytes",
abstract = "Extracellular ATP is among molecules promoting microglia activation and inducing the release of extracellular vesicles (EVs), which are potent mediators of intercellular communication between microglia and the microenvironment. We previously showed that EVs produced under ATP stimulation (ATP-EVs) propagate a robust inflammatory reaction among astrocytes and microglia in vitro and in mice with subclinical neuroinflammation (Verderio et al., 2012). However, the proteome of EVs released upon ATP stimulation has not yet been elucidated. In this study we applied a label free proteomic approach to characterize the proteome of EVs released constitutively and during microglia activation with ATP. We show that ATP drives sorting in EVs of a set of proteins implicated in cell adhesion/extracellular matrix organization, autophagy-lysosomal pathway and cellular metabolism, that may influence the response of recipient astrocytes to EVs. These data provide new clues to molecular mechanisms involved in microglia response to ATP and in microglia signaling to the environment via EVs.",
keywords = "Astrocyte activation, ATP, Extracellular vesicles, Microglia, Proteomics",
author = "Francesco Drago and Marta Lombardi and Ilaria Prada and Martina Gabrielli and Pooja Joshi and Dan Cojoc and Julien Franck and Isabelle Fournier and Jacopo Vizioli and Claudia Verderio",
year = "2017",
month = "12",
day = "13",
doi = "10.3389/fphar.2017.00910",
language = "English",
volume = "8",
journal = "Frontiers in Pharmacology",
issn = "1663-9812",
publisher = "Frontiers Media S.A.",
number = "DEC",

}

TY - JOUR

T1 - ATP modifies the proteome of extracellular vesicles released by microglia and influences their action on astrocytes

AU - Drago, Francesco

AU - Lombardi, Marta

AU - Prada, Ilaria

AU - Gabrielli, Martina

AU - Joshi, Pooja

AU - Cojoc, Dan

AU - Franck, Julien

AU - Fournier, Isabelle

AU - Vizioli, Jacopo

AU - Verderio, Claudia

PY - 2017/12/13

Y1 - 2017/12/13

N2 - Extracellular ATP is among molecules promoting microglia activation and inducing the release of extracellular vesicles (EVs), which are potent mediators of intercellular communication between microglia and the microenvironment. We previously showed that EVs produced under ATP stimulation (ATP-EVs) propagate a robust inflammatory reaction among astrocytes and microglia in vitro and in mice with subclinical neuroinflammation (Verderio et al., 2012). However, the proteome of EVs released upon ATP stimulation has not yet been elucidated. In this study we applied a label free proteomic approach to characterize the proteome of EVs released constitutively and during microglia activation with ATP. We show that ATP drives sorting in EVs of a set of proteins implicated in cell adhesion/extracellular matrix organization, autophagy-lysosomal pathway and cellular metabolism, that may influence the response of recipient astrocytes to EVs. These data provide new clues to molecular mechanisms involved in microglia response to ATP and in microglia signaling to the environment via EVs.

AB - Extracellular ATP is among molecules promoting microglia activation and inducing the release of extracellular vesicles (EVs), which are potent mediators of intercellular communication between microglia and the microenvironment. We previously showed that EVs produced under ATP stimulation (ATP-EVs) propagate a robust inflammatory reaction among astrocytes and microglia in vitro and in mice with subclinical neuroinflammation (Verderio et al., 2012). However, the proteome of EVs released upon ATP stimulation has not yet been elucidated. In this study we applied a label free proteomic approach to characterize the proteome of EVs released constitutively and during microglia activation with ATP. We show that ATP drives sorting in EVs of a set of proteins implicated in cell adhesion/extracellular matrix organization, autophagy-lysosomal pathway and cellular metabolism, that may influence the response of recipient astrocytes to EVs. These data provide new clues to molecular mechanisms involved in microglia response to ATP and in microglia signaling to the environment via EVs.

KW - Astrocyte activation

KW - ATP

KW - Extracellular vesicles

KW - Microglia

KW - Proteomics

UR - http://www.scopus.com/inward/record.url?scp=85038239508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038239508&partnerID=8YFLogxK

U2 - 10.3389/fphar.2017.00910

DO - 10.3389/fphar.2017.00910

M3 - Article

VL - 8

JO - Frontiers in Pharmacology

JF - Frontiers in Pharmacology

SN - 1663-9812

IS - DEC

M1 - 910

ER -