Atrial Natriuretic Peptide (ANP) increases in the mangrove crab Ucides cordatus when exposed to increased environmental salinity

M. Q A Turkin, M. I. Sawaya, M. C F Santos, L. V. Veiga, F. Mantero, G. Opocher

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Abstract

1. 1. Haemolymph taken from the basal joints of the walking legs of the mangrove Brazilian crab Ucides cordatus, was analysed using a very sensitive radioimmunoassay (RIA) technique, before and after specific extraction and purification methods (Vycor-Glass) for immunoreactive atrial natriuretic peptide (IR-ANP) determinations. 2. 2. The mangrove crabs (N = 20) were randomly distributed in four groups, exposed to four different salinities, all of them included in the ecological Brazilian mangrove range: 9, 17, 26, 34%. of seawater. 3. 3. The smallest IR-ANP value, found in the 26‰ salinity (1.25 ± 0.2 pg/ml) was coincident with the isosmotic gradient for haemolymph and external medium, where the crab is not submitted to osmoregulatory disbalances. 4. 4. The IR-ANP values found in the 9 and 17‰ salinity, did not show any significant differences when compared with values obtained at the isosmotic point 26‰. 5. 5. IR-ANP level increased significantly with the external salinity level, from 26 to 34‰ (7.5 ± 5 pg/ml; N = 5). There was a significant positive Kendall Correlation Coefficient (P = 0.035) between the IR-ANP values found at 26 and 34‰ salinity. 6. 6. ANP could participate in the osmoregulation of the Brazilian crab. U. cordatus, contributing towards NaCl extrusion in hyperosmotic environmental salinity.

Original languageEnglish
Pages (from-to)803-806
Number of pages4
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume101
Issue number4
DOIs
Publication statusPublished - 1992

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Salinity
Atrial Natriuretic Factor
Hemolymph
Osmoregulation
Seawater
Walking
Radioimmunoassay
Purification
Glass
Extrusion
Leg
Joints

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

Atrial Natriuretic Peptide (ANP) increases in the mangrove crab Ucides cordatus when exposed to increased environmental salinity. / Turkin, M. Q A; Sawaya, M. I.; Santos, M. C F; Veiga, L. V.; Mantero, F.; Opocher, G.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 101, No. 4, 1992, p. 803-806.

Research output: Contribution to journalArticle

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