Atypical expression and distribution of embryonic stem cell marker, OCT4, in human lung adenocarcinoma

Golnaz Karoubi, Lourdes Cortes-Dericks, Mathias Gugger, Domenico Galetta, Lorenzo Spaggiari, Ralph A. Schmid

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background and Objectives Lung cancer is one of the leading causes of cancer-related deaths in the world. Although the origin still remains to be resolved, a prevailing hypothesis implies the involvement of cancer stem cells (CSCs) responsible for tumor initiation, maintenance, and progression. Embryonic stem cell marker, OCT4, encoding the spliced variants OCT4A and OCT4B, has recently been shown to have a dual role; as a potential adult stem cell marker and as a CSC marker in germline and somatic tumors. Methods We investigated the expression and intracellular distribution of OCT4A and OCT4B using flow cytometry, Western blot and quantitative RT-PCR analyses in normal and lung adenocarcinoma cell lines, primary cultures and tissue biopsies. Results We demonstrate for the presence of rare OCT4A+ and OCT4B+ cells in normal lung. Notably, we observed higher levels of expression and atypical cytoplasmic distribution of OCT4A and not OCT4B, in the malignant setting, strongly indicating an oncogenic role in lung adenocarcinoma. Conclusions We postulate that OCT4A+ cells are involved in the oncogenesis of lung adenocarcinoma. Identification of these cells and the biological processes vital for their subsistence, will guide the development of diagnostic and therapeutic clinical approaches with the goal of eliminating lung adenocarcinoma.

Original languageEnglish
Pages (from-to)689-698
Number of pages10
JournalJournal of Surgical Oncology
Volume102
Issue number6
DOIs
Publication statusPublished - Nov 1 2010

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Embryonic Stem Cells
Neoplastic Stem Cells
Biological Phenomena
Neoplasms
Adult Stem Cells
Lung Neoplasms
Flow Cytometry
Carcinogenesis
Western Blotting
Maintenance
Biopsy
Cell Line
Polymerase Chain Reaction
Lung
Adenocarcinoma of lung
Therapeutics

Keywords

  • adenocarcinoma
  • cancer stem cells
  • lung cancer
  • OCT4A
  • OCT4B

ASJC Scopus subject areas

  • Surgery
  • Oncology

Cite this

Atypical expression and distribution of embryonic stem cell marker, OCT4, in human lung adenocarcinoma. / Karoubi, Golnaz; Cortes-Dericks, Lourdes; Gugger, Mathias; Galetta, Domenico; Spaggiari, Lorenzo; Schmid, Ralph A.

In: Journal of Surgical Oncology, Vol. 102, No. 6, 01.11.2010, p. 689-698.

Research output: Contribution to journalArticle

Karoubi, Golnaz ; Cortes-Dericks, Lourdes ; Gugger, Mathias ; Galetta, Domenico ; Spaggiari, Lorenzo ; Schmid, Ralph A. / Atypical expression and distribution of embryonic stem cell marker, OCT4, in human lung adenocarcinoma. In: Journal of Surgical Oncology. 2010 ; Vol. 102, No. 6. pp. 689-698.
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