Au, Pd and maghemite nanofunctionalized hydroxyapatite scaffolds for bone regeneration

Giovanna Calabrese, Salvatore Petralia, Claudia Fabbi, Stefano Forte, Domenico Franco, Salvatore Guglielmino, Emanuela Esposito, Salvatore Cuzzocrea, Francesco Traina, Sabrina Conoci

Research output: Contribution to journalArticlepeer-review

Abstract

Nanotechnology plays a key role in the development of innovative scaffolds for bone tissue engineering (BTE) allowing the incorporation of nanomaterials able to improve cell proliferation and differentiation. In this study, Mg-HA-Coll type I scaffolds (Mg-HA-based scaffolds) were nanofunctionalized with gold nanorods (Au NRs), palladium nanoparticles (Pd NPs) and maghemite nanoparticles (MAG NPs). Nanofunctionalized Mg-HA-based scaffolds (NF-HA-Ss) were tested for their ability to promote both the proliferation and the differentiation of adipose-derived mesenchymal stem cells (hADSCs). Results clearly highlight that MAG nanofunctionalization substantially improves cell proliferation up to 70% compared with the control (Mg-HA-based scaffold), whereas both Au NRs and Pd NPs nanofunctionalization induce a cell growth inhibition of 94% and 89%, respectively. Similar evidences were found for the osteoinductive properties showing relevant calcium deposits (25% higher than the control) for MAG nanofunctionalization, while a decreasing of cell differentiation (20% lower than the control) for both Au NRs and Pd NPs derivatization. These results are in agreement with previous studies that found cytotoxic effects for both Pd NPs and Au NRs. The excellent improvement of both osteoconductivity and osteoinductivity of the MAG NF-HA-S could be attributed to the high intrinsic magnetic field of superparamagnetic MAG NPs. These findings may pave the way for the development of innovative nanostructured scaffolds for BTE.

Original languageEnglish
Pages (from-to)461-469
Number of pages9
JournalRegenerative Biomaterials
Volume7
Issue number5
DOIs
Publication statusPublished - Aug 27 2020

Keywords

  • Pd nanoparticles
  • bone regeneration
  • gold nanoroads
  • hydroxyapatite scaffold
  • maghemite nanoparticles
  • ssue engineering

Fingerprint

Dive into the research topics of 'Au, Pd and maghemite nanofunctionalized hydroxyapatite scaffolds for bone regeneration'. Together they form a unique fingerprint.

Cite this