Abstract
Recombinant human tumor Necrosis Factor (rHuTNF) produced dose-dependent cytoxicity against human ovarian cancer cells, OSC and OMC, obtained from fresh ascites. A combination of rHuTNF and the topoisomerase II inhibitor, Mitoxantrone, produced dose-dependent synergistic cytotocixity on OSC and OMC cells. When OMC cells were incubated simultaneously for one hour with rHuTNF and Mitoxantrone, increased numbers of DNA single-strands breaks were produced. rHuTNF alone did not induce DNA single-strands breaks. These data are consistent with a role for topoisomerase-linked DNA lesions in the rHuTNF mediated potentiation of killing cells by Mitoxantrone.
Original language | English |
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Pages (from-to) | 1411-1414 |
Number of pages | 4 |
Journal | Anticancer Research |
Volume | 12 |
Issue number | 5 |
Publication status | Published - 1992 |
Keywords
- DNA-damage
- Mitoxantrone-cytotoxicity
- rHuTNF
ASJC Scopus subject areas
- Cancer Research
- Oncology