Abstract
Recombinant human tumor Necrosis Factor (rHuTNF) produced dose-dependent cytoxicity against human ovarian cancer cells, OSC and OMC, obtained from fresh ascites. A combination of rHuTNF and the topoisomerase II inhibitor, Mitoxantrone, produced dose-dependent synergistic cytotocixity on OSC and OMC cells. When OMC cells were incubated simultaneously for one hour with rHuTNF and Mitoxantrone, increased numbers of DNA single-strands breaks were produced. rHuTNF alone did not induce DNA single-strands breaks. These data are consistent with a role for topoisomerase-linked DNA lesions in the rHuTNF mediated potentiation of killing cells by Mitoxantrone.
| Original language | English |
|---|---|
| Pages (from-to) | 1411-1414 |
| Number of pages | 4 |
| Journal | Anticancer Research |
| Volume | 12 |
| Issue number | 5 |
| Publication status | Published - 1992 |
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Keywords
- DNA-damage
- Mitoxantrone-cytotoxicity
- rHuTNF
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Augmentation of antineoplastic effects by the combination of recombinant human tumor necrosis factor and mitoxantrone on primary culture of human ovarian cancer cells. / Cimoli, G.; Valenti, M.; Venturini, M.; Conte, P.; Russo, P.
In: Anticancer Research, Vol. 12, No. 5, 1992, p. 1411-1414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Augmentation of antineoplastic effects by the combination of recombinant human tumor necrosis factor and mitoxantrone on primary culture of human ovarian cancer cells
AU - Cimoli, G.
AU - Valenti, M.
AU - Venturini, M.
AU - Conte, P.
AU - Russo, P.
PY - 1992
Y1 - 1992
N2 - Recombinant human tumor Necrosis Factor (rHuTNF) produced dose-dependent cytoxicity against human ovarian cancer cells, OSC and OMC, obtained from fresh ascites. A combination of rHuTNF and the topoisomerase II inhibitor, Mitoxantrone, produced dose-dependent synergistic cytotocixity on OSC and OMC cells. When OMC cells were incubated simultaneously for one hour with rHuTNF and Mitoxantrone, increased numbers of DNA single-strands breaks were produced. rHuTNF alone did not induce DNA single-strands breaks. These data are consistent with a role for topoisomerase-linked DNA lesions in the rHuTNF mediated potentiation of killing cells by Mitoxantrone.
AB - Recombinant human tumor Necrosis Factor (rHuTNF) produced dose-dependent cytoxicity against human ovarian cancer cells, OSC and OMC, obtained from fresh ascites. A combination of rHuTNF and the topoisomerase II inhibitor, Mitoxantrone, produced dose-dependent synergistic cytotocixity on OSC and OMC cells. When OMC cells were incubated simultaneously for one hour with rHuTNF and Mitoxantrone, increased numbers of DNA single-strands breaks were produced. rHuTNF alone did not induce DNA single-strands breaks. These data are consistent with a role for topoisomerase-linked DNA lesions in the rHuTNF mediated potentiation of killing cells by Mitoxantrone.
KW - DNA-damage
KW - Mitoxantrone-cytotoxicity
KW - rHuTNF
UR - http://www.scopus.com/inward/record.url?scp=0026473128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026473128&partnerID=8YFLogxK
M3 - Article
VL - 12
SP - 1411
EP - 1414
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 5
ER -