Augmentation of GG2EE macrophage cell line-mediated anti-Candida activity by gamma interferon, tumor necrosis factor, and interleukin-1

E. Blasi, S. Farinelli, L. Varesio, F. Bistoni

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Abstract

The expression of anti-Candida activity in the GG2EE macrophage cell line, generated by immortalization of fresh bone marrow with v-raf and v-myc oncogenes, was studied. GG2EE cells spontaneously inhibited the growth of an agerminative mutant of Candida albicans in vitro. The anti-Candida activity was maximal after 8 h of coculture and was proportional to the effector-to-target ratio. Gamma interferon (IFN-γ), interleukin-1 (IL-1), and tumor necrosis factor (TNF) all significantly enhanced the anti-Candida activity of GG2EE cells. In contrast, IL-3, IL-4, and colony-stimulating factor 1 were ineffective. The augmentation of anti-Candida activity was not always concomitant with enhancement of phagocytosis, since IFN-γ and colony-stimulating factor 1, but not IL-1 or TNF, augmented the phagocytic ability of GG2EE cells. Furthermore, the augmentation of anti-Candida activity in GG2EE cells did not correlate with the acquisition of antitumor activity. In fact, none of the cytokines alone were able to induce antitumor activity in GG2EE cells, which, however, could be activated to a tumoricidal stage by IFN-γ plus heat-killed Listeria monocytogenes. These findings demonstrate that GG2EE cells exhibit spontaneous anti-Candida activity and that such activity is enhanced by TNF, IL-1, and IFN-γ.

Original languageEnglish
Pages (from-to)1073-1077
Number of pages5
JournalInfection and Immunity
Volume58
Issue number4
Publication statusPublished - 1990

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Interleukin-1
Candida
Interferon-gamma
Tumor Necrosis Factor-alpha
Macrophages
Cell Line
Interferons
Macrophage Colony-Stimulating Factor
myc Genes
Interleukin-3
Listeria monocytogenes
Coculture Techniques
Candida albicans
Phagocytosis
Interleukin-4
Hot Temperature
Bone Marrow
Cytokines
Growth

ASJC Scopus subject areas

  • Immunology

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Augmentation of GG2EE macrophage cell line-mediated anti-Candida activity by gamma interferon, tumor necrosis factor, and interleukin-1. / Blasi, E.; Farinelli, S.; Varesio, L.; Bistoni, F.

In: Infection and Immunity, Vol. 58, No. 4, 1990, p. 1073-1077.

Research output: Contribution to journalArticle

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AU - Bistoni, F.

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