Augmentation of human monocyte-mediated cytolysis by interferon

J. R. Jett, A. Mantovani, R. B. Herberman

Research output: Contribution to journalArticlepeer-review

Abstract

Human monocytes, separated by either plastic adherence or adherence to microexudate-coated surfaces, from the peripheral blod of most normal donors were shown to have significant cytolytic activity against TU5, a mouse SV 40-transformed target cell. Spontaneous cytolysis ranged from 0 to 32% at a 40:1 effector:target (E:T) ratio. Augmentation of cytolysis was usually seen when human fibroblast interferon (IF) (103-104 units/ml) was cultured with the effector and target cells for the duration of the assay. The mean increase in percentage cytolysis at 40:1 and 20:1 E:T ratios was greater with monocytes obtained by a microexudate method (24.1 and 22.4%) than with monocytes obtained by a plastic adherence method (16.0 and 8.1%). Only a slight augmentation of cytotoxicity was observed when the effector cells were pretreated with IF for 1-hr. The increased levels of cytotoxicity observed when IF was present during the assay did not appear to be due to the toxic effects of IF on the target cells or to a stable increase in the susceptibility of the target cells to lysis.

Original languageEnglish
Pages (from-to)425-434
Number of pages10
JournalCellular Immunology
Volume54
Issue number2
Publication statusPublished - 1980

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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