Augmenter of liver regeneration enhances the success rate of fetal pancreas transplantation in rodents

Gregg A. Adams, Marcello Maestri, Elizabeth C. Squiers, Edward J. Alfrey, Thomas E. Starzl, Donald C. Dafoe

Research output: Contribution to journalArticle

Abstract

Background. Treatment of fetal pancreas (FP) isografts with insulin- like growth factor-I greatly improves the rate of conversion to euglycemia in diabetic rats. Complete knowledge of other factors that may facilitate the engraftment and function of FP in vivo is still embryonic. Augmenter of liver regeneration (ALR) is a newly described polypeptide growth factor found in weanling rat livers. ALR has trophic effects on regenerating liver. We studied the effects of in situ administration of this agent on FP isografts in rats. Methods. Streptozotocin-diabetic Lewis rats (blood glucose >300 mg/dl) received 16 FP isografts transplanted intramuscularly. ALR was delivered from day 1 through day 14, in doses of 40 or 400 ng/kg/d. Animals were followed for 3 months with serial weights and blood glucose monitoring. These animals were compared with those treated with vehicle alone. Results. Of the group treated with ALR at 40 ng/kg/day for 14 days, 89% (eight of nine) were euglycemic (P=0.0003). Of the group treated with ALR at 400 ng/kg/day for 14 days, 88% (seven of eight) were euglycemic (P=0.0007). Of the group treated with vehicle alone, none of the six were euglycemic. Euglycemia is defined here as glucose

Original languageEnglish
Pages (from-to)32-36
Number of pages5
JournalTransplantation
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 15 1998

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ASJC Scopus subject areas

  • Transplantation
  • Immunology

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