Autism susceptibility candidate 2 (Auts2) encodes a nuclear protein expressed in developing brain regions implicated in autism neuropathology

Francesco Bedogni, Rebecca D. Hodge, Branden R. Nelson, Erika A. Frederick, Naoko Shiba, Ray A. Daza, Robert F. Hevner

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Autism susceptibility candidate 2 (Auts2) is a gene associated with autism and mental retardation, whose function is unknown. Expression of Auts2 mRNA and protein were studied in the developing mouse brain by in situ hybridization, immunohistochemistry, and western blotting. Auts2 mRNA was highly expressed in the developing cerebral cortex and cerebellum, regions often affected by neuropathological changes in autism, and a few other brain regions. On embryonic day (E) 12, Auts2 mRNA was expressed in the cortical preplate, where it colocalized with Tbr1, a transcription factor specific for postmitotic projection neurons. From E16 to postnatal day 21, Auts2 was expressed most abundantly in frontal cortex, hippocampus and cerebellum, including Purkinje cells and deep nuclei. High levels of Auts2 were also detected in developing dorsal thalamus, olfactory bulb, inferior colliculus and substantia nigra. Auts2 protein showed similar regional expression patterns as the mRNA. At the cellular level, Auts2 protein was localized in the nuclei of neurons and some neuronal progenitors.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalGene Expression Patterns
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Autistic Disorder
Nuclear Proteins
Brain
Messenger RNA
Cerebellum
Neuropathology
Neurons
Inferior Colliculi
Proteins
Olfactory Bulb
Purkinje Cells
Frontal Lobe
Substantia Nigra
Cell Nucleus
Thalamus
Intellectual Disability
Cerebral Cortex
In Situ Hybridization
Hippocampus
Transcription Factors

Keywords

  • Autism
  • Auts2
  • Gene expression
  • Neurodevelopment
  • Patterning

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Developmental Biology

Cite this

Autism susceptibility candidate 2 (Auts2) encodes a nuclear protein expressed in developing brain regions implicated in autism neuropathology. / Bedogni, Francesco; Hodge, Rebecca D.; Nelson, Branden R.; Frederick, Erika A.; Shiba, Naoko; Daza, Ray A.; Hevner, Robert F.

In: Gene Expression Patterns, Vol. 10, No. 1, 01.2010, p. 9-15.

Research output: Contribution to journalArticle

Bedogni, Francesco ; Hodge, Rebecca D. ; Nelson, Branden R. ; Frederick, Erika A. ; Shiba, Naoko ; Daza, Ray A. ; Hevner, Robert F. / Autism susceptibility candidate 2 (Auts2) encodes a nuclear protein expressed in developing brain regions implicated in autism neuropathology. In: Gene Expression Patterns. 2010 ; Vol. 10, No. 1. pp. 9-15.
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