Autoantibodies to poly(ADP-ribose)polymerase in autoimmune diseases

C. Negri, A. I. Scovassi, A. Cerino, M. Negroni, R. M. Borzì, R. Meliconi, A. Facchini, C. M. Montecucco, G. C B Astaldi Ricotti

Research output: Contribution to journalArticlepeer-review

Abstract

Antibodies to various nuclear proteins are frequently found in sera of patients affected by connective tissue diseases and other autoimmune diseases. We investigated the specificity of circulating autoantibodies to poly(ADP-ribose)polymerase (pADPRP) in different autoimmune and connective tissue diseases: Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), Progressive Systemic Sclerosis (PSS), Sjogren's Syndrome (SS), Undifferen-tiated Connective Tissue Disease (UCTD), Cryptogenic Fibrosing Alveolitis (CFA) and Sarcoidosis. pADPRP was purified from calf thymus. Antibody specificity was detected by ELISA, western blot and enzyme activity precipitation. Positive values (mean O.D. values + 3 S.D. of 36 normal controls) were obtained in 7/15 SLE patients, 1/18 RA patients, 1/30 PSS, 3/14 SS, 0/5 UCTD, 5/21 CFA and 4/25 Sarcoidosis. The positive sera also recognized the pADPRP protein when tested by western blot. Furthermore the enzyme activity was inhibited after immunoprecipitation by some highly positive sera.

Original languageEnglish
Pages (from-to)203-209
Number of pages7
JournalAutoimmunity
Volume6
Issue number3
DOIs
Publication statusPublished - 1990

Keywords

  • Anti nuclear antibodies
  • Connective tissue diseases
  • DNA repair and transcription
  • ELISA
  • Poly(ADP-ribose)polymerase
  • Western Blot

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Autoantibodies to poly(ADP-ribose)polymerase in autoimmune diseases'. Together they form a unique fingerprint.

Cite this