Autoantibody profile of a cohort of 54 Italian patients with linear IgA bullous dermatosis: LAD-1 denoted as a major auto-antigen of the lamina lucida subtype: Acta Dermato-Venereologica

Cutaneous Immunology group of SIDeMaST, E. Cozzani, G. Di Zenzo, G. Gasparini, A. Salemme, A.F. Agnoletti, C. Vassallo, M. Caproni, E. Antiga, A.V. Marzano, R. Cavalli, C. Ocella, C. De Simone, A. Parodi

Research output: Contribution to journalArticlepeer-review

Abstract

Linear immunoglobulin A (IgA) bullous dermatosis (LABD) is characterized by the presence of multiple IgA autoantibodies, and a comparatively lesser number of IgG antibodies, directed against different hemi-desmosomal antigens. The main autoantigens are LAD-1, LABD-97, BP180 and BP230, type VII collagen and laminin 332. The serology of 54 Italian patients with LABD was studied retrospectively using enzyme-linked immunosorbent assay (ELISA), immunoblotting assay, and indirect immunofluorescence on monkey oesophagus and salt-split skin. Among these, indirect immunofluorescence of salt-split skin elicits the grea-test sensitivity. Sixty-three percent of the sera were observed to be positive, with a lamina lucida pattern observed in 48%, a sub-lamina densa pattern in 2% and a mixed pattern in 13% of cases. IgA reactivity to LAD-1 on immunoblotting was found in 52% of sera, to BP180-NC16A by ELISA in 32% and to BP230 in 26%. Only 17% of patients had circulating IgG autoantibo-dies. LAD-1 was determined to be a major autoantigen of the lamina lucida subtype. Combined serological as-says demonstrated a high sensitivity (82%), sugges-ting that this approach could support diagnosis when a biopsy is not feasible or direct immunofluorescence results are negative.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalActa Derm.-Venereol.
Volume100
Issue number4
DOIs
Publication statusPublished - 2020

Keywords

  • BP180
  • BP230
  • Diagnostic sensitivity
  • Humoral immune re-sponse
  • LAD-1
  • Linear IgA bullous disease
  • autoantibody
  • immunoglobulin A antibody
  • immunoglobulin G antibody
  • adolescent
  • adult
  • aged
  • Article
  • case control study
  • Caucasian
  • child
  • cohort analysis
  • enzyme linked immunosorbent assay
  • esophagus
  • female
  • human
  • human tissue
  • humoral immunity
  • immunoblotting
  • immunofluorescence
  • immunoreactivity
  • keratinocyte
  • lamina lucida
  • linear iga bullous dermatosis
  • major clinical study
  • male
  • priority journal
  • retrospective study
  • skin

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