Autoantibody profile of a cohort of 78 Italian patients with mucous membrane pemphigoid: Correlation between reactivity profile and clinical involvement

Emanuele Cozzani, Giovanni di Zenzo, Valentina Calabresi, Marco Carrozzo, Martina Burlando, Lora Longanesi, Amilcare Cerri, Marzia Caproni, Francesco Sera, Emiliano Antiga, Pietro Quaglino, Angelo V. Marzano, Aurora Parodi

Research output: Contribution to journalArticlepeer-review

Abstract

Direct diagnosis of mucous membrane pemphigoid (MMP) is not easy. Circulating autoantibodies targeting bullous pemphigoid antigens of 180 kDa and 230 kDa (BP180 and BP230), α6β4 integrin, laminin 332 and type VII collagen (Col VII) are not always present. The aims of this study were to characterize the humoral immune response of a cohort of Italian patients with MMP, its association with clinical involvement and severity, and to design an algorithm for efficient serological diagnosis. Seventy-eight MMP sera were studied retrospectively by indirect immunofluorescence on salt-split skin, enzymelinked immunosorbent assay (ELISA) and immunoblotting. Indirect immunofluorescence on salt-split skin resulted in the most sensitive approach for diagnosis of MMP. BP180 was the major autoantigen in MMP patients with oral and cutaneous involvement. Significant associations were found between BP180 reactivity and oral and cutaneous localization of the lesions (p = 0.006), and between Col VII positivity and Setterfield severity score (p = 0.020).

Original languageEnglish
Pages (from-to)768-773
Number of pages6
JournalActa Dermato-Venereologica
Volume96
Issue number6
DOIs
Publication statusPublished - Sep 1 2016

Keywords

  • Autoantibody
  • Diagnosis
  • Mucous membrane pemphigoid

ASJC Scopus subject areas

  • Dermatology

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