Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis

Danilo Villalta; E. Girolami; Maria Grazia Alessio; Maria Concetta Sorrentino; Marilina Tampoia; Ignazio Brusca; Massimo Daves; Brunetta Porcelli; Giuseppina Barberio; Mariaelisabetta Conte; Lisa Pantarotto; Nicola Bizzaro; Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy

doi:10.1002/jcla.21813

Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis

Danilo Villalta, E. Girolami, Maria Grazia Alessio, Maria Concetta Sorrentino, Marilina Tampoia, Ignazio Brusca, Massimo Daves, Brunetta Porcelli, Giuseppina Barberio, Mariaelisabetta Conte, Lisa Pantarotto, Nicola Bizzaro, Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy

ISMETT

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: Autoimmune hepatitis (AIH) is a rare condition characterized by the presence of autoantibodies distinctive of type 1 AIH (AIH-1) and type 2 AIH (AIH-2). The aim of this study was to evaluate the autoantibody profile in a cohort of pediatric and adult AIH patients, using both indirect immunofluorescence (IIF) and a new multiplexed line-blot assay.

METHODS: Sera from 63 pediatric and 53 adult AIH patients were tested for antinuclear (ANA), antismooth muscle (SMA), anti-liver kidney microsome 1 (anti-LKM1), anti-liver cytosol 1 (anti-LC1) autoantibodies using IIF methods; for anti-LKM1, anti-LC1, and soluble liver antigen/liver-pancreas (anti-SLA/LP) autoantibodies using the line-blot; for anti-F-actin autoantibodies using IIF both on VSM47 cell-line and on rat intestinal epithelial cells.

RESULTS: AIH-1 was the most common type of AIH in the adult cohort (73.6%), while AIH-2 was the most common AIH in the pediatric cohort (61.9%). Both in adult and pediatric AIH-2 anti-LKM1 were the prevalent autoantibodies. In pediatric AIH-2 anti-LC1 autoantibodies were more frequent than in adult AIH-2 (59 vs. 28.6%), and in 35.9% of cases they were present alone. In 17 patients anti-LC1 autoantibodies were detected only with the line-blot assay. The levels of anti-LKM1 and of anti-LC1 were not different between adult and pediatric AIH, and the overall agreement between the results obtained with the two IIF methods for F-actin detection was 98.8% (CI 95%: 94.4-99.7%).

CONCLUSIONS: The line-blot assay showed a higher sensitivity than IIF for anti-LC1 detection. Anti-LKM1 and anti-LC1 autoantibody levels are not different in adults and children. An almost perfect agreement between the two IIF methods for anti-F-actin detection has been observed.

Original language	English
Pages (from-to)	41-6
Number of pages	6
Journal	Journal of Clinical Laboratory Analysis
Volume	30
Issue number	1
DOIs	https://doi.org/10.1002/jcla.21813
Publication status	Published - Jan 2016

Keywords

Adolescent
Adult
Aged
Animals
Autoantibodies
Cell Line
Child
Child, Preschool
Cohort Studies
Demography
Epithelial Cells
Female
Fluorescent Antibody Technique, Indirect
Hepatitis, Autoimmune
Humans
Male
Middle Aged
Rats
Young Adult
Journal Article

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Villalta, D., Girolami, E., Alessio, M. G., Sorrentino, M. C., Tampoia, M., Brusca, I., Daves, M., Porcelli, B., Barberio, G., Conte, M., Pantarotto, L., Bizzaro, N., & Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy (2016). Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis. Journal of Clinical Laboratory Analysis, 30(1), 41-6. https://doi.org/10.1002/jcla.21813

Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis. / Villalta, Danilo; Girolami, E.; Alessio, Maria Grazia; Sorrentino, Maria Concetta; Tampoia, Marilina; Brusca, Ignazio; Daves, Massimo; Porcelli, Brunetta; Barberio, Giuseppina; Conte, Mariaelisabetta; Pantarotto, Lisa; Bizzaro, Nicola; Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy.

In: Journal of Clinical Laboratory Analysis, Vol. 30, No. 1, 01.2016, p. 41-6.

Research output: Contribution to journal › Article › peer-review

Villalta, D, Girolami, E, Alessio, MG, Sorrentino, MC, Tampoia, M, Brusca, I, Daves, M, Porcelli, B, Barberio, G, Conte, M, Pantarotto, L, Bizzaro, N & Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy 2016, 'Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis', Journal of Clinical Laboratory Analysis, vol. 30, no. 1, pp. 41-6. https://doi.org/10.1002/jcla.21813

Villalta, Danilo ; Girolami, E. ; Alessio, Maria Grazia ; Sorrentino, Maria Concetta ; Tampoia, Marilina ; Brusca, Ignazio ; Daves, Massimo ; Porcelli, Brunetta ; Barberio, Giuseppina ; Conte, Mariaelisabetta ; Pantarotto, Lisa ; Bizzaro, Nicola ; Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy. / Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis. In: Journal of Clinical Laboratory Analysis. 2016 ; Vol. 30, No. 1. pp. 41-6.

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title = "Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis",

abstract = "BACKGROUND: Autoimmune hepatitis (AIH) is a rare condition characterized by the presence of autoantibodies distinctive of type 1 AIH (AIH-1) and type 2 AIH (AIH-2). The aim of this study was to evaluate the autoantibody profile in a cohort of pediatric and adult AIH patients, using both indirect immunofluorescence (IIF) and a new multiplexed line-blot assay.METHODS: Sera from 63 pediatric and 53 adult AIH patients were tested for antinuclear (ANA), antismooth muscle (SMA), anti-liver kidney microsome 1 (anti-LKM1), anti-liver cytosol 1 (anti-LC1) autoantibodies using IIF methods; for anti-LKM1, anti-LC1, and soluble liver antigen/liver-pancreas (anti-SLA/LP) autoantibodies using the line-blot; for anti-F-actin autoantibodies using IIF both on VSM47 cell-line and on rat intestinal epithelial cells.RESULTS: AIH-1 was the most common type of AIH in the adult cohort (73.6%), while AIH-2 was the most common AIH in the pediatric cohort (61.9%). Both in adult and pediatric AIH-2 anti-LKM1 were the prevalent autoantibodies. In pediatric AIH-2 anti-LC1 autoantibodies were more frequent than in adult AIH-2 (59 vs. 28.6%), and in 35.9% of cases they were present alone. In 17 patients anti-LC1 autoantibodies were detected only with the line-blot assay. The levels of anti-LKM1 and of anti-LC1 were not different between adult and pediatric AIH, and the overall agreement between the results obtained with the two IIF methods for F-actin detection was 98.8% (CI 95%: 94.4-99.7%).CONCLUSIONS: The line-blot assay showed a higher sensitivity than IIF for anti-LC1 detection. Anti-LKM1 and anti-LC1 autoantibody levels are not different in adults and children. An almost perfect agreement between the two IIF methods for anti-F-actin detection has been observed.",

keywords = "Adolescent, Adult, Aged, Animals, Autoantibodies, Cell Line, Child, Child, Preschool, Cohort Studies, Demography, Epithelial Cells, Female, Fluorescent Antibody Technique, Indirect, Hepatitis, Autoimmune, Humans, Male, Middle Aged, Rats, Young Adult, Journal Article",

author = "Danilo Villalta and E. Girolami and Alessio, {Maria Grazia} and Sorrentino, {Maria Concetta} and Marilina Tampoia and Ignazio Brusca and Massimo Daves and Brunetta Porcelli and Giuseppina Barberio and Mariaelisabetta Conte and Lisa Pantarotto and Nicola Bizzaro and {Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy}",

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year = "2016",

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doi = "10.1002/jcla.21813",

language = "English",

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TY - JOUR

T1 - Autoantibody Profiling in a Cohort of Pediatric and Adult Patients With Autoimmune Hepatitis

AU - Villalta, Danilo

AU - Girolami, E.

AU - Alessio, Maria Grazia

AU - Sorrentino, Maria Concetta

AU - Tampoia, Marilina

AU - Brusca, Ignazio

AU - Daves, Massimo

AU - Porcelli, Brunetta

AU - Barberio, Giuseppina

AU - Conte, Mariaelisabetta

AU - Pantarotto, Lisa

AU - Bizzaro, Nicola

AU - Study Group on Autoimmune Diseases of the Italian Society of Laboratory Medicine, Italy

PY - 2016/1

Y1 - 2016/1

N2 - BACKGROUND: Autoimmune hepatitis (AIH) is a rare condition characterized by the presence of autoantibodies distinctive of type 1 AIH (AIH-1) and type 2 AIH (AIH-2). The aim of this study was to evaluate the autoantibody profile in a cohort of pediatric and adult AIH patients, using both indirect immunofluorescence (IIF) and a new multiplexed line-blot assay.METHODS: Sera from 63 pediatric and 53 adult AIH patients were tested for antinuclear (ANA), antismooth muscle (SMA), anti-liver kidney microsome 1 (anti-LKM1), anti-liver cytosol 1 (anti-LC1) autoantibodies using IIF methods; for anti-LKM1, anti-LC1, and soluble liver antigen/liver-pancreas (anti-SLA/LP) autoantibodies using the line-blot; for anti-F-actin autoantibodies using IIF both on VSM47 cell-line and on rat intestinal epithelial cells.RESULTS: AIH-1 was the most common type of AIH in the adult cohort (73.6%), while AIH-2 was the most common AIH in the pediatric cohort (61.9%). Both in adult and pediatric AIH-2 anti-LKM1 were the prevalent autoantibodies. In pediatric AIH-2 anti-LC1 autoantibodies were more frequent than in adult AIH-2 (59 vs. 28.6%), and in 35.9% of cases they were present alone. In 17 patients anti-LC1 autoantibodies were detected only with the line-blot assay. The levels of anti-LKM1 and of anti-LC1 were not different between adult and pediatric AIH, and the overall agreement between the results obtained with the two IIF methods for F-actin detection was 98.8% (CI 95%: 94.4-99.7%).CONCLUSIONS: The line-blot assay showed a higher sensitivity than IIF for anti-LC1 detection. Anti-LKM1 and anti-LC1 autoantibody levels are not different in adults and children. An almost perfect agreement between the two IIF methods for anti-F-actin detection has been observed.

AB - BACKGROUND: Autoimmune hepatitis (AIH) is a rare condition characterized by the presence of autoantibodies distinctive of type 1 AIH (AIH-1) and type 2 AIH (AIH-2). The aim of this study was to evaluate the autoantibody profile in a cohort of pediatric and adult AIH patients, using both indirect immunofluorescence (IIF) and a new multiplexed line-blot assay.METHODS: Sera from 63 pediatric and 53 adult AIH patients were tested for antinuclear (ANA), antismooth muscle (SMA), anti-liver kidney microsome 1 (anti-LKM1), anti-liver cytosol 1 (anti-LC1) autoantibodies using IIF methods; for anti-LKM1, anti-LC1, and soluble liver antigen/liver-pancreas (anti-SLA/LP) autoantibodies using the line-blot; for anti-F-actin autoantibodies using IIF both on VSM47 cell-line and on rat intestinal epithelial cells.RESULTS: AIH-1 was the most common type of AIH in the adult cohort (73.6%), while AIH-2 was the most common AIH in the pediatric cohort (61.9%). Both in adult and pediatric AIH-2 anti-LKM1 were the prevalent autoantibodies. In pediatric AIH-2 anti-LC1 autoantibodies were more frequent than in adult AIH-2 (59 vs. 28.6%), and in 35.9% of cases they were present alone. In 17 patients anti-LC1 autoantibodies were detected only with the line-blot assay. The levels of anti-LKM1 and of anti-LC1 were not different between adult and pediatric AIH, and the overall agreement between the results obtained with the two IIF methods for F-actin detection was 98.8% (CI 95%: 94.4-99.7%).CONCLUSIONS: The line-blot assay showed a higher sensitivity than IIF for anti-LC1 detection. Anti-LKM1 and anti-LC1 autoantibody levels are not different in adults and children. An almost perfect agreement between the two IIF methods for anti-F-actin detection has been observed.

KW - Adolescent

KW - Adult

KW - Aged

KW - Animals

KW - Autoantibodies

KW - Cell Line

KW - Child

KW - Child, Preschool

KW - Cohort Studies

KW - Demography

KW - Epithelial Cells

KW - Female

KW - Fluorescent Antibody Technique, Indirect

KW - Hepatitis, Autoimmune

KW - Humans

KW - Male

KW - Middle Aged

KW - Rats

KW - Young Adult

KW - Journal Article

U2 - 10.1002/jcla.21813

DO - 10.1002/jcla.21813

M3 - Article

C2 - 25242745

VL - 30

SP - 41

EP - 46

JO - Journal of Clinical Laboratory Analysis

JF - Journal of Clinical Laboratory Analysis

SN - 0887-8013

IS - 1

ER -