Autofluorescence properties of rat liver under hypermetabolic conditions

Anna Cleta Croce, Uliana De Simone, Mariapia Vairetti, Andrea Ferrigno, Giovanni Bottiroli

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Autofluorescence response to oxygen supply modulation has been investigated in livers of rats under the hypermetabolic state associated to a pathological condition - hyperthyroidism - that is known to enhance hepatocyte metabolic activities involving both NAD, i.e. oxidative pathways engaged in ATP synthesis, and NADP, i.e. reductive bio-synthesis and antioxidant functions. Experiments have been performed on rats in normal condition or submitted to long-term thyroxine (T4) administration. Histological inspection did not show any appreciable morphological alteration in liver parenchyma; biochemical analysis indicated an increase in both NADP+ and NADPH contents. Autofluorescence properties have been monitored in vivo, via a fiber optic probe, on exposed livers both during induction of global ischemia and after restoration of blood circulation. Alteration of oxygen supply modulated liver autofluorescence properties, mainly as to NAD(P)H contribution, in dependence of changes in pyridine coenzymes redox state. With respect to euthyroid, hyperthyroid rat livers exhibited higher autofluorescence signals in all phases of the experiment, and a faster signal decay time upon reoxygenation. The results have been interpreted on the basis of a larger content of NADPH - the coenzyme not directly oxidized in respiratory processes and likely providing an almost constant autofluorescence background contribution - and of uncoupling effects facilitating the respiratory NADH oxidation, associated with the hyperthyroid condition. The results obtained in the liver hypermetabolic model provide interesting perspectives for a further improvement of the diagnostic implications of autofluorescence.

Original languageEnglish
Pages (from-to)1202-1209
Number of pages8
JournalPhotochemical and Photobiological Sciences
Volume6
Issue number11
DOIs
Publication statusPublished - 2007

Fingerprint

liver
Liver
rats
Rats
NADP
Hyperthyroidism
NAD
coenzymes
Oxygen supply
Coenzymes
thyroxine
blood circulation
Oxygen
ischemia
biosynthesis
adenosine triphosphate
Blood Circulation
Biosynthesis
antioxidants
Hemodynamics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Autofluorescence properties of rat liver under hypermetabolic conditions. / Croce, Anna Cleta; De Simone, Uliana; Vairetti, Mariapia; Ferrigno, Andrea; Bottiroli, Giovanni.

In: Photochemical and Photobiological Sciences, Vol. 6, No. 11, 2007, p. 1202-1209.

Research output: Contribution to journalArticle

Croce, Anna Cleta ; De Simone, Uliana ; Vairetti, Mariapia ; Ferrigno, Andrea ; Bottiroli, Giovanni. / Autofluorescence properties of rat liver under hypermetabolic conditions. In: Photochemical and Photobiological Sciences. 2007 ; Vol. 6, No. 11. pp. 1202-1209.
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