Autogeneic but Not Allogeneic Earthworm Effector Coelomocytes Kill the Mammalian Tumor Cell Target K562

EDWIN L. COOPER, ANDREA COSSARIZZA, MICHAEL M. SUZUKI, STEFANO SALVIOLI, MIRIAM CAPRI, DANIELA QUAGLINO, CLAUDIO FRANCESCHI

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Abstract

Earthworm coelomocytes have been used as effector cells against the human tumor target, K562. To first assess the viability of effectors, incorporation of [3H]thymidine was tested and was higher in autogeneic (A ⇔ A, self) than in allogeneic (A ⇔ B, nonself) coelomocytes. A ⇔ A showed significantly greater numbers in S, G2, or M phases than A ⇔ B coelomocytes. When A ⇔ A or A ⇔ B were cultured, no significant cell killing occurred in either, as measured in a 4-hr51Cr release assay. A ⇔ A but not A ⇔ B killed K562 target cells. Cytotoxicity was dependent upon membrane binding between small, electron-dense coelomocytes and targets; it was enhanced by adding PHA. The heat labile supernatant from A ⇔ A but not from A ⇔ B killed K562 targets after cultivation for 10 min at 22°C, but not immediately after washing. Recognition of, binding to, and killing of foreign cells in a natural killer cell-like reaction may reflect natural immunity in earthworms.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalCellular Immunology
Volume166
Issue number1
DOIs
Publication statusPublished - Nov 1995

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ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

COOPER, EDWIN. L., COSSARIZZA, ANDREA., SUZUKI, MICHAEL. M., SALVIOLI, STEFANO., CAPRI, MIRIAM., QUAGLINO, DANIELA., & FRANCESCHI, CLAUDIO. (1995). Autogeneic but Not Allogeneic Earthworm Effector Coelomocytes Kill the Mammalian Tumor Cell Target K562. Cellular Immunology, 166(1), 113-122. https://doi.org/10.1006/cimm.1995.0013