TY - JOUR
T1 - Autogeneic but Not Allogeneic Earthworm Effector Coelomocytes Kill the Mammalian Tumor Cell Target K562
AU - COOPER, EDWIN L.
AU - COSSARIZZA, ANDREA
AU - SUZUKI, MICHAEL M.
AU - SALVIOLI, STEFANO
AU - CAPRI, MIRIAM
AU - QUAGLINO, DANIELA
AU - FRANCESCHI, CLAUDIO
PY - 1995/11
Y1 - 1995/11
N2 - Earthworm coelomocytes have been used as effector cells against the human tumor target, K562. To first assess the viability of effectors, incorporation of [3H]thymidine was tested and was higher in autogeneic (A ⇔ A, self) than in allogeneic (A ⇔ B, nonself) coelomocytes. A ⇔ A showed significantly greater numbers in S, G2, or M phases than A ⇔ B coelomocytes. When A ⇔ A or A ⇔ B were cultured, no significant cell killing occurred in either, as measured in a 4-hr51Cr release assay. A ⇔ A but not A ⇔ B killed K562 target cells. Cytotoxicity was dependent upon membrane binding between small, electron-dense coelomocytes and targets; it was enhanced by adding PHA. The heat labile supernatant from A ⇔ A but not from A ⇔ B killed K562 targets after cultivation for 10 min at 22°C, but not immediately after washing. Recognition of, binding to, and killing of foreign cells in a natural killer cell-like reaction may reflect natural immunity in earthworms.
AB - Earthworm coelomocytes have been used as effector cells against the human tumor target, K562. To first assess the viability of effectors, incorporation of [3H]thymidine was tested and was higher in autogeneic (A ⇔ A, self) than in allogeneic (A ⇔ B, nonself) coelomocytes. A ⇔ A showed significantly greater numbers in S, G2, or M phases than A ⇔ B coelomocytes. When A ⇔ A or A ⇔ B were cultured, no significant cell killing occurred in either, as measured in a 4-hr51Cr release assay. A ⇔ A but not A ⇔ B killed K562 target cells. Cytotoxicity was dependent upon membrane binding between small, electron-dense coelomocytes and targets; it was enhanced by adding PHA. The heat labile supernatant from A ⇔ A but not from A ⇔ B killed K562 targets after cultivation for 10 min at 22°C, but not immediately after washing. Recognition of, binding to, and killing of foreign cells in a natural killer cell-like reaction may reflect natural immunity in earthworms.
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U2 - 10.1006/cimm.1995.0013
DO - 10.1006/cimm.1995.0013
M3 - Article
C2 - 7585971
AN - SCOPUS:0028856745
VL - 166
SP - 113
EP - 122
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 1
ER -