Autografting Ph-negative blood precursor cells in chronic myeloid leukaemia

F. Frassoni, D. Giordano, M. Podestà, G. Piaggio, F. Benvenuto, A. Pitto, O. Figari, A. Dejana, E. Lerma, F. Vassallo, M. Sessarego, A. Carella

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The study was devised to evaluate whether it was possible to collect Philadelphia-negative precursor cells in patients with chronic myeloid leukaemia. The approach was based on previous experience showing that complete remission (Ph-negative bone narrow cells) is rarely achieved after chemotherapy and is very short-lasting. We decided to explore whether it was possible to collect Ph-negative precursor cells in peripheral blood during the early phase of haemopoietic recovery. These data show that: the collection of Ph-negative precursor cells occurred in 12/16 (75%) patients mobilized within one year of diagnosis (group A) versus 12/33 (36%) in patients with a history of more than one year of disease (group B). Furthermore the numbers of Ph-negative precursor cells were significantly much higher at diagnosis. Ten patients mobilized at diagnosis were subsequently autografted with such Ph-negative precursor cells. Five of them remain Ph-negative from 4 to 12 months while the other five have percentages of Ph-positive cells in their marrow ranging from 20% to 70%. In this stage of the disease the procedure is safe and associated with a very good compliance. Occasional restoration of Ph-negative haemopoiesis could be observed up to 40 months after autograft, in patients of group B, but most of patients revert to Ph-positive haemopoiesis. In conclusion these data suggest that it is possible to restore Ph-negative haemopoieis in 70% of patients mobilized at diagnosis. This percentage represent the highest one can obtain without allogeneic BMT, and this includes patients who never would have been cytogenetic responders to IFN-α. Whether and how long for Ph-negative status can be maintained is a matter for future observation and study.

Original languageEnglish
JournalBone Marrow Transplantation
Issue numberSUPPL. 3
Publication statusPublished - May 1996


  • Autografting
  • Chronic myeloid leukaemia
  • Haemopoietis progenitor cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation


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