Autologous peripheral blood stem cell transplantation for myocardial regeneration: A novel strategy for cell collection and surgical injection

Giulio Pompilio, Aldo Cannata, Fedro Peccatori, Francesco Bertolini, Angelo Nascimbene, Maurizio C. Capogrossi, Paolo Biglioli

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose Bone-marrow and peripheral blood-derived stem cells can be used as stimulators of myogenesis and angiogenesis. We describe an original technique for collection and surgical intramyocardial injection of peripheral blood-derived stem cells. Description Stem cells are mobilized from the bone marrow by means of subcutaneous administration of Lenogastrim (Granocyte 34 [Aventis Pharma, Milan, Italy]) for 4 days. Then the day before the operation the peripheral blood-derived stem cells are collected by means of apheresis and processed in order to obtain the CD 133+ cells. Cells are injected into the myocardium in a beating heart in order to induce angiogenesis locally or myogenesis, or both. When necessary, off-pump coronary artery bypass grafting is previously accomplished. Evaluation Thus far we have investigated 4 patients (3 patients who have received off-pump peripheral blood stem cell injection and coronary bypass grafting through median sternotomies, and 1 patient who underwent cell transplant alone through a minimally-invasive transdiaphragmatic approach). No complications were noted at a mean of 4 months after surgery. Conclusions This novel method of peripheral bone marrow stem cell collection and intramyocardial injection seems to be safe, feasible, and reproducible. However, there is need of further evidence to definitely assess safety issues and clinical results.

Original languageEnglish
Pages (from-to)1808-1812
Number of pages5
JournalAnnals of Thoracic Surgery
Volume78
Issue number5
DOIs
Publication statusPublished - Nov 2004

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

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