Automated mononuclear cell collection: a feasibility study employing a new software for extracorporeal photopheresis

Claudia Del Fante, Luigia Scudeller, Cristina Mortellaro, Gianluca Viarengo, Alberto Martinasso, Cesare Perotti

Research output: Contribution to journalArticlepeer-review


Background and objectives: Very recently, Fresenius Kabi, improved the software (autoMNC lymphocytes, SW 04.03.08) for mononuclear cells (MNCs) collection with the aim to ameliorate the quality of harvest, employing the automated autoMNC lymphocytes software SW 04.03.09. Herein, we report the results of an observational study evaluating the feasibility of MNCs collection in patients undergoing extracorporeal photopheresis (ECP) at our centre, using the new COM.TEC software 04.03.08c for MNC collection, afterwards integrated in the software 04.03.09, available on the market since November 2018. Materials and methods: Thirty adult patients (21 males and 9 females) with GvHD, Chronic Lung Allograft Dysfunction or renal rejection, were consecutively enrolled to undergo 1 ECP procedure by the offline technique, according to our internal protocol, processing 1·5 blood volumes. Feasibility of collection was defined as: Hct in collection bag ≤5%, MNCs purity (percentage of MNCs/bag) ≥80%, MNCs collection efficiency (CE2) ≥60%, patient's platelet depletion ≤50%. Results: Thirty ECP procedures were evaluated. Feasibility (defined by the four parameters previously described) of MNCs collection was observed in 1 out of the 30 harvests analysed. Median Hct in the product was 3·45% (IQR: 2·6–5·0), and median MNCs purity was 97·2% (IQR 89·1–98·6). Median CE2 for MNCs was 21·4% (IQR: 11·9–41·2), and median patient's platelet depletion was 36·2% (IQR 21·9–51·4). Conclusion: The autoMNC lymphocytes software SW 04.03.08c for MNCs collection in ECP setting demonstrated to collect a good quality product in terms of purity and RBC contamination even if the collection efficiency and platelet contamination must be improved.

Original languageEnglish
JournalVox Sanguinis
Publication statusPublished - Jan 1 2019


  • automated mononuclear cell collection
  • extracorporeal photopheresis
  • off-line ECP

ASJC Scopus subject areas

  • Hematology


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