Abstract
Background and objectives: Very recently, Fresenius Kabi, improved the software (autoMNC lymphocytes, SW 04.03.08) for mononuclear cells (MNCs) collection with the aim to ameliorate the quality of harvest, employing the automated autoMNC lymphocytes software SW 04.03.09. Herein, we report the results of an observational study evaluating the feasibility of MNCs collection in patients undergoing extracorporeal photopheresis (ECP) at our centre, using the new COM.TEC software 04.03.08c for MNC collection, afterwards integrated in the software 04.03.09, available on the market since November 2018. Materials and methods: Thirty adult patients (21 males and 9 females) with GvHD, Chronic Lung Allograft Dysfunction or renal rejection, were consecutively enrolled to undergo 1 ECP procedure by the offline technique, according to our internal protocol, processing 1·5 blood volumes. Feasibility of collection was defined as: Hct in collection bag ≤5%, MNCs purity (percentage of MNCs/bag) ≥80%, MNCs collection efficiency (CE2) ≥60%, patient's platelet depletion ≤50%. Results: Thirty ECP procedures were evaluated. Feasibility (defined by the four parameters previously described) of MNCs collection was observed in 1 out of the 30 harvests analysed. Median Hct in the product was 3·45% (IQR: 2·6–5·0), and median MNCs purity was 97·2% (IQR 89·1–98·6). Median CE2 for MNCs was 21·4% (IQR: 11·9–41·2), and median patient's platelet depletion was 36·2% (IQR 21·9–51·4). Conclusion: The autoMNC lymphocytes software SW 04.03.08c for MNCs collection in ECP setting demonstrated to collect a good quality product in terms of purity and RBC contamination even if the collection efficiency and platelet contamination must be improved.
| Original language | English |
|---|---|
| Journal | Vox Sanguinis |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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Keywords
- automated mononuclear cell collection
- extracorporeal photopheresis
- off-line ECP
ASJC Scopus subject areas
- Hematology
Cite this
Automated mononuclear cell collection : a feasibility study employing a new software for extracorporeal photopheresis. / Del Fante, Claudia; Scudeller, Luigia; Mortellaro, Cristina; Viarengo, Gianluca; Martinasso, Alberto; Perotti, Cesare.
In: Vox Sanguinis, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Automated mononuclear cell collection
T2 - a feasibility study employing a new software for extracorporeal photopheresis
AU - Del Fante, Claudia
AU - Scudeller, Luigia
AU - Mortellaro, Cristina
AU - Viarengo, Gianluca
AU - Martinasso, Alberto
AU - Perotti, Cesare
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background and objectives: Very recently, Fresenius Kabi, improved the software (autoMNC lymphocytes, SW 04.03.08) for mononuclear cells (MNCs) collection with the aim to ameliorate the quality of harvest, employing the automated autoMNC lymphocytes software SW 04.03.09. Herein, we report the results of an observational study evaluating the feasibility of MNCs collection in patients undergoing extracorporeal photopheresis (ECP) at our centre, using the new COM.TEC software 04.03.08c for MNC collection, afterwards integrated in the software 04.03.09, available on the market since November 2018. Materials and methods: Thirty adult patients (21 males and 9 females) with GvHD, Chronic Lung Allograft Dysfunction or renal rejection, were consecutively enrolled to undergo 1 ECP procedure by the offline technique, according to our internal protocol, processing 1·5 blood volumes. Feasibility of collection was defined as: Hct in collection bag ≤5%, MNCs purity (percentage of MNCs/bag) ≥80%, MNCs collection efficiency (CE2) ≥60%, patient's platelet depletion ≤50%. Results: Thirty ECP procedures were evaluated. Feasibility (defined by the four parameters previously described) of MNCs collection was observed in 1 out of the 30 harvests analysed. Median Hct in the product was 3·45% (IQR: 2·6–5·0), and median MNCs purity was 97·2% (IQR 89·1–98·6). Median CE2 for MNCs was 21·4% (IQR: 11·9–41·2), and median patient's platelet depletion was 36·2% (IQR 21·9–51·4). Conclusion: The autoMNC lymphocytes software SW 04.03.08c for MNCs collection in ECP setting demonstrated to collect a good quality product in terms of purity and RBC contamination even if the collection efficiency and platelet contamination must be improved.
AB - Background and objectives: Very recently, Fresenius Kabi, improved the software (autoMNC lymphocytes, SW 04.03.08) for mononuclear cells (MNCs) collection with the aim to ameliorate the quality of harvest, employing the automated autoMNC lymphocytes software SW 04.03.09. Herein, we report the results of an observational study evaluating the feasibility of MNCs collection in patients undergoing extracorporeal photopheresis (ECP) at our centre, using the new COM.TEC software 04.03.08c for MNC collection, afterwards integrated in the software 04.03.09, available on the market since November 2018. Materials and methods: Thirty adult patients (21 males and 9 females) with GvHD, Chronic Lung Allograft Dysfunction or renal rejection, were consecutively enrolled to undergo 1 ECP procedure by the offline technique, according to our internal protocol, processing 1·5 blood volumes. Feasibility of collection was defined as: Hct in collection bag ≤5%, MNCs purity (percentage of MNCs/bag) ≥80%, MNCs collection efficiency (CE2) ≥60%, patient's platelet depletion ≤50%. Results: Thirty ECP procedures were evaluated. Feasibility (defined by the four parameters previously described) of MNCs collection was observed in 1 out of the 30 harvests analysed. Median Hct in the product was 3·45% (IQR: 2·6–5·0), and median MNCs purity was 97·2% (IQR 89·1–98·6). Median CE2 for MNCs was 21·4% (IQR: 11·9–41·2), and median patient's platelet depletion was 36·2% (IQR 21·9–51·4). Conclusion: The autoMNC lymphocytes software SW 04.03.08c for MNCs collection in ECP setting demonstrated to collect a good quality product in terms of purity and RBC contamination even if the collection efficiency and platelet contamination must be improved.
KW - automated mononuclear cell collection
KW - extracorporeal photopheresis
KW - off-line ECP
UR - http://www.scopus.com/inward/record.url?scp=85071305475&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071305475&partnerID=8YFLogxK
U2 - 10.1111/vox.12841
DO - 10.1111/vox.12841
M3 - Article
AN - SCOPUS:85071305475
JO - Vox Sanguinis
JF - Vox Sanguinis
SN - 0042-9007
ER -