Automated red blood cell depletion in ABO incompatible grafts in the pediatric setting

Claudia Del Fante, Luigia Scudeller, Santina Recupero, Gianluca Viarengo, Stella Boghen, Antonella Gurrado, Marco Zecca, Jerard Seghatchian, Cesare Perotti

Research output: Contribution to journalReview article

Abstract

Bone marrow ABO incompatible transplantations require graft manipulation prior to infusion to avoid potentially lethal side effects. We analyzed the influence of pre-manipulation factors (temperature at arrival, transit time, time of storage at 4 °C until processing and total time from collection to red blood cell depletion) on the graft quality of 21 red blood cell depletion procedures in ABO incompatible pediatric transplants. Bone marrow collections were processed using the Spectra Optia® (Terumo BCT) automated device. Temperature at arrival ranged between 4 °C and 6 °C, median transit time was 9.75 h (range 0.33–28), median time of storage at 4°–6 °C until processing was 1.8 h (range 0.41–18.41) and median time from collection to RBC depletion was 21 h (range1–39.4). Median percentage of red blood cell depletion was 97.7 (range 95.4–98.5), median mononuclear cells recovery was 92.2% (range 40–121.2), median CD34+ cell recovery was 93% (range 69.9–161.2), median cell viability was 97.7% (range 94–99.3) and median volume reduction was 83.9% (range 82–92). Graft quality was not significantly different between BM units </> median age. Our preliminary data show that when all good manifacturing practices are respected the post-manipulation graft quality is excellent also for those units processed after 24 h.

Original languageEnglish
Pages (from-to)895-899
Number of pages5
JournalTransfusion and Apheresis Science
Volume56
Issue number6
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

Keywords

  • ABO incompatibility
  • Allogeneic haematopoietic stem cell transplantation
  • Pediatric stem cell transplantation
  • Red blood cell depletion

ASJC Scopus subject areas

  • Hematology

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