Automatic quantitation of cell colonies on petri dishes by computerized image analysis

Alberto Bollea, Marco Masseroli, Antonino Nicotra, Patrizia Beccaglia, Soumitra Sen, Eugenio Erba, Giovanna Balconi

Research output: Contribution to journalArticlepeer-review


Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agents in vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.

Original languageEnglish
Pages (from-to)185-191
Number of pages7
Issue number3
Publication statusPublished - Oct 1995


  • antineoplastic drugs
  • clonogenicity
  • colony assay
  • Image analysis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Biotechnology


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