Abstract
Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agents in vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.
| Original language | English |
|---|---|
| Pages (from-to) | 185-191 |
| Number of pages | 7 |
| Journal | Cytotechnology |
| Volume | 17 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 1995 |
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Keywords
- antineoplastic drugs
- clonogenicity
- colony assay
- Image analysis
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Biotechnology
Cite this
Automatic quantitation of cell colonies on petri dishes by computerized image analysis. / Bollea, Alberto; Masseroli, Marco; Nicotra, Antonino; Beccaglia, Patrizia; Sen, Soumitra; Erba, Eugenio; Balconi, Giovanna.
In: Cytotechnology, Vol. 17, No. 3, 10.1995, p. 185-191.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Automatic quantitation of cell colonies on petri dishes by computerized image analysis
AU - Bollea, Alberto
AU - Masseroli, Marco
AU - Nicotra, Antonino
AU - Beccaglia, Patrizia
AU - Sen, Soumitra
AU - Erba, Eugenio
AU - Balconi, Giovanna
PY - 1995/10
Y1 - 1995/10
N2 - Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agents in vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.
AB - Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agents in vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.
KW - antineoplastic drugs
KW - clonogenicity
KW - colony assay
KW - Image analysis
UR - http://www.scopus.com/inward/record.url?scp=0029189624&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029189624&partnerID=8YFLogxK
U2 - 10.1007/BF00749656
DO - 10.1007/BF00749656
M3 - Article
AN - SCOPUS:0029189624
VL - 17
SP - 185
EP - 191
JO - Cytotechnology
JF - Cytotechnology
SN - 0920-9069
IS - 3
ER -