Automatic quantitation of cell colonies on petri dishes by computerized image analysis

Alberto Bollea; Marco Masseroli; Antonino Nicotra; Patrizia Beccaglia; Soumitra Sen; Eugenio Erba; Giovanna Balconi

doi:10.1007/BF00749656

Automatic quantitation of cell colonies on petri dishes by computerized image analysis

Alberto Bollea, Marco Masseroli, Antonino Nicotra, Patrizia Beccaglia, Soumitra Sen, Eugenio Erba, Giovanna Balconi

Istituto di Ricerche Farmacologiche "Mario Negri"

Research output: Contribution to journal › Article

Abstract

Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agents in vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.

Original language	English
Pages (from-to)	185-191
Number of pages	7
Journal	Cytotechnology
Volume	17
Issue number	3
DOIs	https://doi.org/10.1007/BF00749656
Publication status	Published - Oct 1995

Keywords

antineoplastic drugs
clonogenicity
colony assay
Image analysis

ASJC Scopus subject areas

Clinical Biochemistry
Cell Biology
Biotechnology

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10.1007/BF00749656

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Bollea, A., Masseroli, M., Nicotra, A., Beccaglia, P., Sen, S., Erba, E., & Balconi, G. (1995). Automatic quantitation of cell colonies on petri dishes by computerized image analysis. Cytotechnology, 17(3), 185-191. https://doi.org/10.1007/BF00749656

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abstract = "Clonogenic assay is one of the most sensitive assays, widely used to evaluate the effects of antineoplastic agents in vitro. A computer program was developed on an IBAS 2.0 Image Analysis System for automated quantiation of cell colonies and clone area on Petri dishes. The sensitivity of the clonogenic assay can be greatly increased by evaluating the mean area of the clones. The program gives an objective, accurate and fast evaluation of large samples. It is simple to use and offers a high degree of flexibility. Special algorithms and techniques have been implemented for good quantitation of both connected and well-separated colonies and to reduce the background noise and the general error rate. The principles and solutions presented are applicable to any other image analysis system.",

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author = "Alberto Bollea and Marco Masseroli and Antonino Nicotra and Patrizia Beccaglia and Soumitra Sen and Eugenio Erba and Giovanna Balconi",

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AU - Bollea, Alberto

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AU - Nicotra, Antonino

AU - Beccaglia, Patrizia

AU - Sen, Soumitra

AU - Erba, Eugenio

AU - Balconi, Giovanna

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