Automatic quantitative evaluation of autoradiographic band films by computerized image analysis

Marco Masseroli, Andrea Messori, Caterina Bendotti, Mauro Ponti, Gianluigi Forloni

Research output: Contribution to journalArticle

Abstract

The present paper describes a new image processing method for automatic quantitative analysis of autoradiographic band films. It was developed in a specific image analysis environment (IBAS 2.0), but the algorithms and methods can be utilized elsewhere. The program is easy to use and presents some particularly useful features for evaluation of autoradiographic band films, such as the choice of whole film or single lane background determination; the possibility of evaluating bands with film scratch artifacts and the quantification in absolute terms or relative to reference values. The method was tested by comparison with laser-scanner densitometric quantifications of the same autoradiograms. The results show the full compatibility of the two methods and demonstrate the reliability and sensitivity of image analysis. The method can be used not only to evaluate autoradiographic band films, but to analyze any type of signal bands on other materials (e.g. electrophoresis gel, chromatographic paper, etc.).

Original languageEnglish
JournalLife Sciences
Volume53
Issue number20
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Pharmacology

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