Automation of human sperm cell analysis by flow cytometry

Fulvio Ferrara, Rita Daverio, Giuliano Mazzini, Pierangelo Bonini, Giuseppe Banfi

Research output: Contribution to journalArticle

Abstract

Semen sample analysis is routinely performed by microscopical evaluation and manual techniques by laboratory operators; the analysis is affected by a wide imprecision related to variability among observers, influencing its clinical validity. Our aim was to automate sperm analysis with the use of flow cytometry for evaluation of cell counts and typing and with the use of a new membrane-permeant nucleic acid stain for evaluation of sperm viability. Statistical analysis of the comparison between manual and automated methods for sperm counts was performed by the Bland and Altman method; the mean difference was 0.243 x 106 sperms/mL. The precision of the flow cytometric analysis was evaluated with whole sperm; the between-run CV was 7.5% and the within-run CV was 2.5%. Data observed suggest that flow cytometric sperm analysis, with high precision and accuracy and low costs, can be proposed for routine use in clinical laboratories.

Original languageEnglish
Pages (from-to)801-807
Number of pages7
JournalClinical Chemistry
Volume43
Issue number5
Publication statusPublished - 1997

Keywords

  • semen analysis
  • vital staining

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Ferrara, F., Daverio, R., Mazzini, G., Bonini, P., & Banfi, G. (1997). Automation of human sperm cell analysis by flow cytometry. Clinical Chemistry, 43(5), 801-807.