Automation of o-dianisidine assay for ceruloplasmin activity analyses: Usefulness of investigation in Wilson’s disease and in hepatic encephalopathy

Mariacristina Siotto, Patrizio Pasqualetti, Massimo Marano, Rosanna Squitti

Research output: Contribution to journalArticle

Abstract

Ceruloplasmin (Cp) is a serum ferroxidase that plays an essential role in iron metabolism. It is routinely tested by immunoturbidimetric assays that quantify the concentration of the protein both in its active and inactive forms. Cp activity is generally analyzed manually; the process is time-consuming, has a limited repeatability, and is not suitable for a clinical setting. To overcome these inconveniences, we have set the automation of the o-dianisidine Cp activity assay on a Cobas Mira Plus apparatus. The automation was rapid and repeatable, and the data were provided in terms of IU/L. The assay was adapted for human sera and showed a good precision [coefficient of variation (CV) 3.7 %] and low limit of detection (LoD 11.58 IU/L). The simultaneous analysis of Cp concentration and activity in the same run allowed us to calculate the Cp-specific activity that provides a better index of the overall Cp status. To test the usefulness of this automation, we tested this assay on 104 healthy volunteers and 36 patients with Wilson’s disease, hepatic encephalopathy, and chronic liver disease. Cp activity and specific activity distinguished better patients between groups with respect to Cp concentration alone, and providing support for the clinical investigation of neurological diseases in which liver failure is one of the clinical hallmarks.

Original languageEnglish
Pages (from-to)1281-1286
Number of pages6
JournalJournal of Neural Transmission
Volume121
Issue number10
DOIs
Publication statusPublished - Mar 25 2014

Keywords

  • Ceruloplasmin
  • Ceruloplasmin activity
  • Hepatic diseases
  • Hepatic encephalopathy
  • Neurodegenerative diseases
  • Wilson’s disease

ASJC Scopus subject areas

  • Biological Psychiatry
  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Medicine(all)

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