Autophagy plays an important role in the containment of HIV-1 in nonprogressor-infected patients

Research output: Contribution to journalArticle

Abstract

Recent in vitro studies have suggested that autophagy may play a role in both HIV-1 replication and disease progression. In this study we investigated whether autophagy protects the small proportion of HIV-1 infected individuals who remain clinically stable for years in the absence of antiretroviral therapy, these named long-term nonprogressors (LTNP) and elite controllers (EC). We found that peripheral blood mononuclear cells (PBMC) of the HIV-1 controllers present a significantly higher amount of autophagic vesicles associated with an increased expression of autophagic markers with respect to normal progressors. Of note, ex vivo treatment of PBMC from the HIV-1 controllers with the MTOR inhibitor rapamycin results in a more efficient autophagic response, leading to a reduced viral production. These data lead us to propose that autophagy contributes to limiting viral pathogenesis in HIV-1 controllers by targeting viral components for degradation.

Original languageEnglish
Pages (from-to)1167-1178
Number of pages12
JournalAutophagy
Volume10
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint

HIV Long-Term Survivors
Autophagy
HIV-1
Blood Cells
Viral Structures
Sirolimus
Disease Progression
Therapeutics

Keywords

  • AMBRA1
  • ATG5
  • Autophagy
  • BECN1
  • Cell death
  • Elite controllers
  • HIV-1
  • Long-term nonprogressors

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

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title = "Autophagy plays an important role in the containment of HIV-1 in nonprogressor-infected patients",
abstract = "Recent in vitro studies have suggested that autophagy may play a role in both HIV-1 replication and disease progression. In this study we investigated whether autophagy protects the small proportion of HIV-1 infected individuals who remain clinically stable for years in the absence of antiretroviral therapy, these named long-term nonprogressors (LTNP) and elite controllers (EC). We found that peripheral blood mononuclear cells (PBMC) of the HIV-1 controllers present a significantly higher amount of autophagic vesicles associated with an increased expression of autophagic markers with respect to normal progressors. Of note, ex vivo treatment of PBMC from the HIV-1 controllers with the MTOR inhibitor rapamycin results in a more efficient autophagic response, leading to a reduced viral production. These data lead us to propose that autophagy contributes to limiting viral pathogenesis in HIV-1 controllers by targeting viral components for degradation.",
keywords = "AMBRA1, ATG5, Autophagy, BECN1, Cell death, Elite controllers, HIV-1, Long-term nonprogressors",
author = "Roberta Nardacci and Alessandra Amendola and Fabiola Ciccosanti and Marco Corazzari and Valentina Esposito and Chrysoula Vlassi and Chiara Taibi and Fimia, {Gian Maria} and {Del Nonno}, Franca and Giuseppe Ippolito and Gianpiero D'Offizi and Mauro Piacentini",
year = "2014",
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AU - Nardacci, Roberta

AU - Amendola, Alessandra

AU - Ciccosanti, Fabiola

AU - Corazzari, Marco

AU - Esposito, Valentina

AU - Vlassi, Chrysoula

AU - Taibi, Chiara

AU - Fimia, Gian Maria

AU - Del Nonno, Franca

AU - Ippolito, Giuseppe

AU - D'Offizi, Gianpiero

AU - Piacentini, Mauro

PY - 2014

Y1 - 2014

N2 - Recent in vitro studies have suggested that autophagy may play a role in both HIV-1 replication and disease progression. In this study we investigated whether autophagy protects the small proportion of HIV-1 infected individuals who remain clinically stable for years in the absence of antiretroviral therapy, these named long-term nonprogressors (LTNP) and elite controllers (EC). We found that peripheral blood mononuclear cells (PBMC) of the HIV-1 controllers present a significantly higher amount of autophagic vesicles associated with an increased expression of autophagic markers with respect to normal progressors. Of note, ex vivo treatment of PBMC from the HIV-1 controllers with the MTOR inhibitor rapamycin results in a more efficient autophagic response, leading to a reduced viral production. These data lead us to propose that autophagy contributes to limiting viral pathogenesis in HIV-1 controllers by targeting viral components for degradation.

AB - Recent in vitro studies have suggested that autophagy may play a role in both HIV-1 replication and disease progression. In this study we investigated whether autophagy protects the small proportion of HIV-1 infected individuals who remain clinically stable for years in the absence of antiretroviral therapy, these named long-term nonprogressors (LTNP) and elite controllers (EC). We found that peripheral blood mononuclear cells (PBMC) of the HIV-1 controllers present a significantly higher amount of autophagic vesicles associated with an increased expression of autophagic markers with respect to normal progressors. Of note, ex vivo treatment of PBMC from the HIV-1 controllers with the MTOR inhibitor rapamycin results in a more efficient autophagic response, leading to a reduced viral production. These data lead us to propose that autophagy contributes to limiting viral pathogenesis in HIV-1 controllers by targeting viral components for degradation.

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KW - ATG5

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KW - Cell death

KW - Elite controllers

KW - HIV-1

KW - Long-term nonprogressors

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