Autoradiographical analysis of excitatory amino acid binding sites in rat hippocampus during the development of hippocampal kindling

A. Vezzani, R. Serafini, R. Samanin, A. C. Foster

Research output: Contribution to journalArticle

Abstract

Binding sites for excitatory amino acids have been determined by autoradiographical procedures in the rat hippocampus and striatum during hippocampal kindling. The binding sites measured were the N-methyl-d-aspartate (NMDA)-sensitive sites for l-[3H]glutamate and [3H]MK-801 sites (transmitter recognition site and ion channel of the NMDA receptor, respectively), [3H]α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) sites (quisqualate receptor), [3H]kainate sites (kainate receptor) and NMDA-insensitive sites for l-[3H]glutamate. In general, little change was apparent in the hippocampus or striatum for any of these binding sites when assessed 48 h after attaining stages 1 2, 3 or 5 of kindling. These results suggest that hippocampal kindling does not bring about a change in the excitatory amino acid receptor binding sites examined, and that the appearance of an NMDA receptor-mediated component to synaptic responses in the hippocampus produced by kindling15, cannot be explained on this basis.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalBrain Research
Volume526
Issue number1
DOIs
Publication statusPublished - Aug 27 1990

Keywords

  • Epilepsy
  • Kainate receptor
  • MK-801
  • N-Methyl-d-aspartate receptor
  • Quisqualate receptor

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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