IL SISTEMA BASATO SUL COMPLESSO AVIDINA-BIOTINA APPLICATO ALLE TECNICHE DI FLUORESCENZA IN IMMUNOPATOLOGIA CUTANEA

Translated title of the contribution: Avidin-biotin complex applied to fluorescence techniques in cutaneous immunopathology

Research output: Contribution to journalArticle

Abstract

The use of avidin-biotin complex (ABC) applied to immunofluorescence (IF) techniques theoretically improves sensitivity and specificity of IF tests. In this paper, the authors report the results obtained comparing biotinilated anti-human IgG and biotinilated protein A, stained with fluorescinated avidin, with antisera employed in usual IF techniques. 16 skin specimens coming from patients with pemphigus, bullous pemphigoid, eosinophilic spongiosis, epidermolysis bullosa acquisita and systemic lupus erythematosus were tested. ABC has revealed a better sensibility with the same specificity than usual techniques giving, because of higher dilution, very clean specimens. Biotinilated anti-human IgG links aspecifically to dermal structures making diagnosis difficult when the immunoglobulins' deposits are at the dermal epidermal junction. On the contrary biotinilated protein A does not link to dermal structures and immunodeposits at the dermal epidermal junction are very easily detected. The use of ABC in dermatological diagnosis could be very useful because of its sensibility, that can display pathological reports, doubtful with standard techniques. Moreover the use of ABC in IF techniques is cheaper than usual IF methods, because it employs more diluted sera.

Original languageItalian
Pages (from-to)537-542
Number of pages6
JournalGiornale Italiano di Dermatologia e Venereologia
Volume116
Issue number12
Publication statusPublished - 1981

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Avidin
Biotin
Fluorescent Antibody Technique
Fluorescence
Skin
Staphylococcal Protein A
Epidermolysis Bullosa Acquisita
Bullous Pemphigoid
Pemphigus
Systemic Lupus Erythematosus
Immunoglobulins
Immune Sera
Sensitivity and Specificity
Serum

ASJC Scopus subject areas

  • Dermatology

Cite this

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title = "IL SISTEMA BASATO SUL COMPLESSO AVIDINA-BIOTINA APPLICATO ALLE TECNICHE DI FLUORESCENZA IN IMMUNOPATOLOGIA CUTANEA",
abstract = "The use of avidin-biotin complex (ABC) applied to immunofluorescence (IF) techniques theoretically improves sensitivity and specificity of IF tests. In this paper, the authors report the results obtained comparing biotinilated anti-human IgG and biotinilated protein A, stained with fluorescinated avidin, with antisera employed in usual IF techniques. 16 skin specimens coming from patients with pemphigus, bullous pemphigoid, eosinophilic spongiosis, epidermolysis bullosa acquisita and systemic lupus erythematosus were tested. ABC has revealed a better sensibility with the same specificity than usual techniques giving, because of higher dilution, very clean specimens. Biotinilated anti-human IgG links aspecifically to dermal structures making diagnosis difficult when the immunoglobulins' deposits are at the dermal epidermal junction. On the contrary biotinilated protein A does not link to dermal structures and immunodeposits at the dermal epidermal junction are very easily detected. The use of ABC in dermatological diagnosis could be very useful because of its sensibility, that can display pathological reports, doubtful with standard techniques. Moreover the use of ABC in IF techniques is cheaper than usual IF methods, because it employs more diluted sera.",
author = "M. Monti and E. Berti and G. Carminati",
year = "1981",
language = "Italian",
volume = "116",
pages = "537--542",
journal = "Minerva dermatologica",
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AU - Berti, E.

AU - Carminati, G.

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N2 - The use of avidin-biotin complex (ABC) applied to immunofluorescence (IF) techniques theoretically improves sensitivity and specificity of IF tests. In this paper, the authors report the results obtained comparing biotinilated anti-human IgG and biotinilated protein A, stained with fluorescinated avidin, with antisera employed in usual IF techniques. 16 skin specimens coming from patients with pemphigus, bullous pemphigoid, eosinophilic spongiosis, epidermolysis bullosa acquisita and systemic lupus erythematosus were tested. ABC has revealed a better sensibility with the same specificity than usual techniques giving, because of higher dilution, very clean specimens. Biotinilated anti-human IgG links aspecifically to dermal structures making diagnosis difficult when the immunoglobulins' deposits are at the dermal epidermal junction. On the contrary biotinilated protein A does not link to dermal structures and immunodeposits at the dermal epidermal junction are very easily detected. The use of ABC in dermatological diagnosis could be very useful because of its sensibility, that can display pathological reports, doubtful with standard techniques. Moreover the use of ABC in IF techniques is cheaper than usual IF methods, because it employs more diluted sera.

AB - The use of avidin-biotin complex (ABC) applied to immunofluorescence (IF) techniques theoretically improves sensitivity and specificity of IF tests. In this paper, the authors report the results obtained comparing biotinilated anti-human IgG and biotinilated protein A, stained with fluorescinated avidin, with antisera employed in usual IF techniques. 16 skin specimens coming from patients with pemphigus, bullous pemphigoid, eosinophilic spongiosis, epidermolysis bullosa acquisita and systemic lupus erythematosus were tested. ABC has revealed a better sensibility with the same specificity than usual techniques giving, because of higher dilution, very clean specimens. Biotinilated anti-human IgG links aspecifically to dermal structures making diagnosis difficult when the immunoglobulins' deposits are at the dermal epidermal junction. On the contrary biotinilated protein A does not link to dermal structures and immunodeposits at the dermal epidermal junction are very easily detected. The use of ABC in dermatological diagnosis could be very useful because of its sensibility, that can display pathological reports, doubtful with standard techniques. Moreover the use of ABC in IF techniques is cheaper than usual IF methods, because it employs more diluted sera.

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