AvidinOX™ for highly efficient tissue-pretargeted radionuclide therapy

Rita De Santis, Barbara Leoni, Antonio Rosi, Claudio Albertoni, Guido Forni, Rodica Cojoca, Manuela Iezzi, Piero Musiani, Giovanni Paganelli, Marco Chinol, Paolo Carminati

Research output: Contribution to journalArticle

Abstract

Avidin is widely used in vitro for its capacity to bind biotin. However, avidińs in vivo use is limited by its short residence in blood and tissues. An avidin variant, named AvidinOX,™ has been recently described. This product is obtained by 4-hydroxyazobenzene-2-carboxylic acid-assisted sodium periodate oxidation of avidin. This method generates aldehyde groups from avidin carbohydrates, sparing biotin-binding sites from inactivation. AvidinOX binds cellular and interstitial protein amino groups through Schiff's bases, resulting in a tissue half-life of 2 weeks, compared with 2 hours of native avidin. Binding of AvidinOX occurs in normal and neoplastic tissues. Data show that AvidinOX, administered intranipple in the breast of transgenic BALB/neuT mice, is highly efficient for capturing 90Y-biotinDOTA, intravenously injected after 48 hours, leading to eradication of multifocal cancer lesions. Efficacy data, together with good tolerability results, indicate that AvidinOX is a highly innovative reagent for tissue-pretargeted radionuclide therapy.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalCancer Biotherapy and Radiopharmaceuticals
Volume25
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

Keywords

  • ™cancer
  • AvidinOX
  • Pretargeting
  • Radionuclide therapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Pharmacology
  • Medicine(all)

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  • Cite this

    De Santis, R., Leoni, B., Rosi, A., Albertoni, C., Forni, G., Cojoca, R., Iezzi, M., Musiani, P., Paganelli, G., Chinol, M., & Carminati, P. (2010). AvidinOX™ for highly efficient tissue-pretargeted radionuclide therapy. Cancer Biotherapy and Radiopharmaceuticals, 25(2), 143-148. https://doi.org/10.1089/cbr.2009.0738