Avoiding Ethanol Presence in DNA Samples Enhances the Performance of Ultraviolet Resonance Raman Spectroscopy Analysis

Francesca Cammisuli, Lorella Pascolo, Marcello Morgutti, Alessandro Gessini, Claudio Masciovecchio, Francesco D'Amico

Research output: Contribution to journalArticlepeer-review

Abstract

Ethanol is an essential chemical reagent in DNA preparation as its use increases the yield of extraction. All methodologies for DNA isolation involve the use of ethanol in order to prevent DNA dissolution in water and to optimize the binding of DNA to chromatographic membranes. In this note, we show how the presence of ethanol traces in DNA aqueous solution affects ultraviolet Raman spectra, leading to possible misinterpretations. We report a simple method to remove the ethanol Raman features from the spectra, based on heating the DNA sample at 80 °C, followed by a slow cooling procedure.

Original languageEnglish
Pages (from-to)152-155
Number of pages4
JournalApplied Spectroscopy
Volume71
Issue number1
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • DNA
  • ethanol
  • QIAamp DNA Mini Kit
  • QIAsymphony DSP DNA midi kit
  • ultraviolet resonance Raman spectroscopy

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

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