B-b10 (Anti-CD25)-saporin immunotoxin—a possible tool in graft-versus-host disease treatment1

Pier Luigi Tazzari, Andrea Bolognesi, Daniela De Totero, Stefano Pileri, Roberto Conte, John Wijdenes, Patrick Herve, Marco Soria, Fiorenzo Stirpe, Marco Gobbi

Research output: Contribution to journalArticlepeer-review

Abstract

An immunotoxin containing the B-B10 MoAb, directed against the CD25 determinant, and the ribosome-inactivating protein saporin, inhibits 3H-TdR incorporation in phytohemagglutin, allogeneic-stimulated lymphocytes (primary and secondary mixed-lymphocyte reaction), and in an alloreactive T cell clone. A lower degree of inhibition was obtained with the B-B10 MoAb, which is known to inhibit IL-2 activity, as well as with the unconjugated compounds. These results suggest that the in vivo administration of the conjugate might be a more effective tool in the treatment of patients affected by graft-versus-host disease than B-B10 alone, by inducing an efficient killing of allogeneic-reacting T lymphocytes.

Original languageEnglish
Pages (from-to)351-366
Number of pages16
JournalTransplantation
Volume54
Issue number2
Publication statusPublished - 1992

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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