B-b10 (Anti-CD25)-saporin immunotoxin—a possible tool in graft-versus-host disease treatment1

Pier Luigi Tazzari; Andrea Bolognesi; Daniela De Totero; Stefano Pileri; Roberto Conte; John Wijdenes; Patrick Herve; Marco Soria; Fiorenzo Stirpe; Marco Gobbi

B-b10 (Anti-CD25)-saporin immunotoxin—a possible tool in graft-versus-host disease treatment1

Pier Luigi Tazzari, Andrea Bolognesi, Daniela De Totero, Stefano Pileri, Roberto Conte, John Wijdenes, Patrick Herve, Marco Soria, Fiorenzo Stirpe, Marco Gobbi

Research output: Contribution to journal › Article › peer-review

Abstract

An immunotoxin containing the B-B10 MoAb, directed against the CD25 determinant, and the ribosome-inactivating protein saporin, inhibits 3H-TdR incorporation in phytohemagglutin, allogeneic-stimulated lymphocytes (primary and secondary mixed-lymphocyte reaction), and in an alloreactive T cell clone. A lower degree of inhibition was obtained with the B-B10 MoAb, which is known to inhibit IL-2 activity, as well as with the unconjugated compounds. These results suggest that the in vivo administration of the conjugate might be a more effective tool in the treatment of patients affected by graft-versus-host disease than B-B10 alone, by inducing an efficient killing of allogeneic-reacting T lymphocytes.

Original language	English
Pages (from-to)	351-366
Number of pages	16
Journal	Transplantation
Volume	54
Issue number	2
Publication status	Published - 1992

ASJC Scopus subject areas

Transplantation
Immunology

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B-b10 (Anti-CD25)-saporin immunotoxin—a possible tool in graft-versus-host disease treatment1. / Tazzari, Pier Luigi; Bolognesi, Andrea; De Totero, Daniela; Pileri, Stefano; Conte, Roberto; Wijdenes, John; Herve, Patrick; Soria, Marco; Stirpe, Fiorenzo; Gobbi, Marco.

In: Transplantation, Vol. 54, No. 2, 1992, p. 351-366.

Research output: Contribution to journal › Article › peer-review

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abstract = "An immunotoxin containing the B-B10 MoAb, directed against the CD25 determinant, and the ribosome-inactivating protein saporin, inhibits 3H-TdR incorporation in phytohemagglutin, allogeneic-stimulated lymphocytes (primary and secondary mixed-lymphocyte reaction), and in an alloreactive T cell clone. A lower degree of inhibition was obtained with the B-B10 MoAb, which is known to inhibit IL-2 activity, as well as with the unconjugated compounds. These results suggest that the in vivo administration of the conjugate might be a more effective tool in the treatment of patients affected by graft-versus-host disease than B-B10 alone, by inducing an efficient killing of allogeneic-reacting T lymphocytes.",

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AU - Bolognesi, Andrea

AU - De Totero, Daniela

AU - Pileri, Stefano

AU - Conte, Roberto

AU - Wijdenes, John

AU - Herve, Patrick

AU - Soria, Marco

AU - Stirpe, Fiorenzo

AU - Gobbi, Marco

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AB - An immunotoxin containing the B-B10 MoAb, directed against the CD25 determinant, and the ribosome-inactivating protein saporin, inhibits 3H-TdR incorporation in phytohemagglutin, allogeneic-stimulated lymphocytes (primary and secondary mixed-lymphocyte reaction), and in an alloreactive T cell clone. A lower degree of inhibition was obtained with the B-B10 MoAb, which is known to inhibit IL-2 activity, as well as with the unconjugated compounds. These results suggest that the in vivo administration of the conjugate might be a more effective tool in the treatment of patients affected by graft-versus-host disease than B-B10 alone, by inducing an efficient killing of allogeneic-reacting T lymphocytes.

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B-b10 (Anti-CD25)-saporin immunotoxin—a possible tool in graft-versus-host disease treatment1

Abstract

ASJC Scopus subject areas

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