B-Cell depletion improves islet allograft survival with anti-CD45RB

Kang Mi Lee, Heidi Yeh, Gaoping Zhao, Lingling Wei, Matthew O'Connor, Ryan T. Stott, Julie Soohoo, Kyri Dunussi, Paolo Fiorina, Shaoping Deng, James F. Markmann, James I. Kim

Research output: Contribution to journalArticlepeer-review

Abstract

A short course of anti-CD45RB leads to long-term islet allograft survival and donor-specific tolerance in approximately half of immunocompetent mice. We have previously demonstrated that anti-CD45RB antibodymediated tolerance requires B-cells for cardiac allograft survival. We therefore asked whether B-cells were also required for anti-CD45RB antibody-mediated survival of islets. Unexpectedly, we found that nearly 100% of islet allografts survive long term in B-cell-deficient mice. Similarly, B-cell depletion by anti-CD22/cal augmented anti-CD45RB-mediated tolerance when administered pretransplant, although it had no effect on tolerance induction when administered posttransplant. Our results demonstrate that the role of B-cells in promoting tolerance with anti-CD45RB is graft specific, promoting tolerance in cardiac grafts but resisting tolerance in islet transplantation. These findings may help elucidate the varied action of B-cells in promoting tolerance versus rejection. Ó 2014 Cognizant Comm. Corp.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalCell Transplantation
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 2014

Keywords

  • Anti-CD22
  • Anti-CD45RB
  • B-cells
  • Islet transplantation
  • Tolerance

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

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