B cell development in the spleen takes place in discrete steps and is determined by the quality of B cell receptor-derived signals

Florienne Loder, Bettina Mutschler, Robert J. Ray, Christopher J. Paige, Paschalis Sideras, Raul Torres, Marinus C. Lamers, Rita Carsetti

Research output: Contribution to journalArticle

Abstract

Only mature B lymphocytes can enter the lymphoid follicles of spleen and lymph nodes and thus efficiently participate in the immune response. Mature, long-lived B lymphocytes derive from short-lived precursors generated in the bone marrow. We show that selection into the mature pool is an active process and takes place in the spleen. Two populations of splenic B cell were identified as precursors for mature B cells. Transitional B cells of type 1 (T1) are recent immigrants from the bone marrow. They develop into the transitional B cells of type 2 (T2), which are cycling and found exclusively in the primary follicles of the spleen. Mature B cells can be generated fro T1 or T2 B cells. Mice with genetic deletions of elements participating in the B cell receptor signaling cascade display developmental arrest at the T1 or T2 stage. The analysis of these defects showed that the development of T2 and mature B cells from T1 precursors requires defined qualitative and quantitative signals derived from the B cell receptor and that the induction of longevity and maturation requires different signals.

Original languageEnglish
Pages (from-to)75-89
Number of pages15
JournalJournal of Experimental Medicine
Volume190
Issue number1
DOIs
Publication statusPublished - Jul 5 1999

Keywords

  • B cell development
  • Bruton's tyrosine kinase
  • CD45
  • Spleen
  • Transitional B cells

ASJC Scopus subject areas

  • Immunology

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