B cell helper assays.

Sergio Abrignani, Elena Tonti, Giulia Casorati, Paolo Dellabona

Research output: Contribution to journalArticle

Abstract

Activation, proliferation and differentiation of naïve B lymphocytes into memory B cells and plasma cells requires engagement of the B cell receptor (BCR) coupled to T-cell help (1, 2). T cells deliver help in cognate fashion when they are activated upon recognition of specific MHC-peptide complexes presented by B cells. T cells can also deliver help in a non-cognate or bystander fashion, when they do not find specific MHC-peptide complexes on B cells and are activated by alternative mechanisms. T-cell dependent activation of B cells can be studied in vitro by experimental models called "B cell helper assays" that are based on the co-culture of B cells with activated T cells. These assays allow to decipher the molecular bases for productive T-dependent B cell responses. We show here examples of B cell helper assays in vitro, which can be reproduced with any subset of T lymphocytes that displays the appropriate helper signals.

Original languageEnglish
Pages (from-to)15-26
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume514
Publication statusPublished - 2009

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B-Lymphocytes
T-Lymphocytes
Peptides
T-Lymphocyte Subsets
Coculture Techniques
Plasma Cells
Theoretical Models

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

B cell helper assays. / Abrignani, Sergio; Tonti, Elena; Casorati, Giulia; Dellabona, Paolo.

In: Methods in molecular biology (Clifton, N.J.), Vol. 514, 2009, p. 15-26.

Research output: Contribution to journalArticle

Abrignani, Sergio ; Tonti, Elena ; Casorati, Giulia ; Dellabona, Paolo. / B cell helper assays. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 514. pp. 15-26.
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