B-cell subset alterations and correlated factors in HIV-1 infection

Simone Pensieroso, Laura Galli, Silvia Nozza, Nicolas Ruffin, Antonella Castagna, Giuseppe Tambussi, Bo Hejdeman, Donatella Misciagna, Agostino Riva, Mauro Malnati, Francesca Chiodi, Gabriella Scarlatti

Research output: Contribution to journalArticlepeer-review


Objectives: During HIV-1 infection, the development, phenotype, and functionality of B cells are impaired. Transitional B cells and aberrant B-cell populations arise in blood, whereas a declined percentage of resting memory B cells is detected. Our study aimed at pinpointing the demographic, immunological, and viral factors driving these pathological findings, and the role of antiretroviral therapy in reverting these alterations. Design: B-cell phenotype and correlating factors were evaluated. Methods: Variations in B-cell subsets were evaluated by flow cytometry in HIV-1-infected individuals naive to therapy, elite controllers, and patients treated with antiretroviral drugs (virological control or failure). Multivariable analysis was performed to identify variables independently associated with the B-cell alterations. Results: Significant differences were observed among patients' groups in relation to all B-cell subsets. Resting memory B cells were preserved in patients naive to therapy and elite controllers, but reduced in treated patients. Individuals naive to therapy and experiencing multidrug failure, as well as elite controllers, had significantly higher levels of activated memory B cells compared to healthy controls. In the multivariate analysis, plasma viral load and nadir CD4+ T cells independently correlated with major B-cell alterations. Coinfection with hepatitis C but not hepatitis B virus also showed an impact on specific B-cell subsets. Successful protracted antiretroviral treatment led to normalization of all B-cell subsets with exception of resting memory B cells. Conclusion: Our results indicate that viremia and nadir CD4+ T cells are important prognostic markers of B-cell perturbations and provide evidence that resting memory B-cell depletion during chronic infection is not reverted upon successful antiretroviral therapy.

Original languageEnglish
Pages (from-to)1209-1217
Number of pages9
JournalAIDS (London, England)
Issue number8
Publication statusPublished - May 15 2013


  • B cells
  • CD4 T-cell nadir
  • Coinfection
  • Elite controller
  • HIV-1
  • Viremia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases


Dive into the research topics of 'B-cell subset alterations and correlated factors in HIV-1 infection'. Together they form a unique fingerprint.

Cite this