B cells from chronic lymphocytic leukemia (CLL) patients are strong inducers of proliferation and major histocompatibility complex (MHC)-unrestricted [natural killer (NK)-like] cytotoxicity in normal T-lymphocytes

Lina Matera, Robert Foa, Massimo Massaia, Mirella Giovarelli, Fabrizio Veglia, Alessandra Cesano, Paolo Lusso, Alberto Piazza, Daniela Santoli

Research output: Contribution to journalArticle

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Abstract

We studied the ability of chronic lymphocytic leukemia (CLL) B cells to stimulate proliferation and major histocompatibility complex (MHC)-unrestricted [natural killer (NK)-like] cytotoxicity in a mixed lymphocyte tumor-cell culture (MLTC). CLL-derived B cells induced a significantly higher proliferative response than did B cells from healthy normal donors. Comparable levels of interferon and interleukin-2 production were detected in the mixed lymphocyte cultures (MLC) set up with normal B cells from healthy donors and in MLTC. Higher levels of NK-like cytotoxic activity were induced after stimulation with CLL than with normal B cells in nonlytic precursors. Inhibition experiments with specific monoclonal antibodies indicate that the NK-like response in MLTC is attributable to HLA class II antigens, which are expressed at comparable levels on CLL and normal B cells. Percoll gradient centrifugation of the MLC and MLTC recovered cells demonstrated that the NK-like effectors in both types of cultures were blast-transformed, highly mitotic cells.

Original languageEnglish
Pages (from-to)329-337
Number of pages9
JournalJournal of Clinical Immunology
Volume9
Issue number4
DOIs
Publication statusPublished - Jul 1989

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B-Cell Chronic Lymphocytic Leukemia
Major Histocompatibility Complex
Lymphocytes
T-Lymphocytes
Cell Culture Techniques
B-Lymphocytes
Neoplasms
Tissue Donors
Histocompatibility Antigens Class II
HLA Antigens
Centrifugation
Natural Killer Cells
Interferons
Interleukin-2
Monoclonal Antibodies

Keywords

  • Chronic lymphocytic leukemia
  • mixed lymphocyte culture
  • mixed lymphocyte tumor-cell culture
  • natural killer (NK)-like cytotoxic activity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

B cells from chronic lymphocytic leukemia (CLL) patients are strong inducers of proliferation and major histocompatibility complex (MHC)-unrestricted [natural killer (NK)-like] cytotoxicity in normal T-lymphocytes. / Matera, Lina; Foa, Robert; Massaia, Massimo; Giovarelli, Mirella; Veglia, Fabrizio; Cesano, Alessandra; Lusso, Paolo; Piazza, Alberto; Santoli, Daniela.

In: Journal of Clinical Immunology, Vol. 9, No. 4, 07.1989, p. 329-337.

Research output: Contribution to journalArticle

Matera, Lina ; Foa, Robert ; Massaia, Massimo ; Giovarelli, Mirella ; Veglia, Fabrizio ; Cesano, Alessandra ; Lusso, Paolo ; Piazza, Alberto ; Santoli, Daniela. / B cells from chronic lymphocytic leukemia (CLL) patients are strong inducers of proliferation and major histocompatibility complex (MHC)-unrestricted [natural killer (NK)-like] cytotoxicity in normal T-lymphocytes. In: Journal of Clinical Immunology. 1989 ; Vol. 9, No. 4. pp. 329-337.
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