B lymphocyte-restricted expression of prion protein does not enable prion replication in prion protein knockout mice

Fabio Montrasio, Antonio Cozzio, Eckhard Flechsig, Daniela Rossi, Michael A. Klein, Thomas Rülicke, Alex J. Raeber, Christian A J Vosshenrich, Juliane Proft, Adriano Aguzzi, Charles Weissmann

Research output: Contribution to journalArticlepeer-review

Abstract

Prion replication in spleen and neuroinvasion after i.p. inoculation of mice is impaired in forms of immunodeficiency where mature B lymphocytes are lacking. In spleens of wild-type mice, infectivity is associated with B and T lymphocytes and stroma but not with circulating lymphocytes. We generated transgenic priorn protein knockout mice overexpressing prion protein in B lymphocytes and found that they failed to accumulate prions in spleen after i.p. inoculation. We conclude that splenic B lymphocytes are not prion-replication competent and that they acquire prions from other cells, most likely follicular dendritic cells with which they closely associate and whose maturation depends on them.

Original languageEnglish
Pages (from-to)4034-4037
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number7
DOIs
Publication statusPublished - Mar 27 2001

ASJC Scopus subject areas

  • Genetics
  • General

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