B lymphocytes in humans express ZAP-70 when activated in vivo

Giovanna Cutrona, Monica Colombo, Serena Matis, Daniele Reverberi, Mariella Dono, Vicenzo Tarantino, Nicholas Chiorazzi, Manlio Ferrarini

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

ZAP-70 is a protein tyrosine kinase initially found in T and NK cells. Recently, this important signaling element was detected in leukemic B cells from a subgroup of patients with B cell chronic lymphocytic leukemia (B-CLL). In this study, ZAP-70 was detected in normal B cells from human tonsils, but not from peripheral blood. The cDNA sequence of B cell ZAP-70 was the same as that in T cells. Germinal center B cells and plasma cells had a substantial proportion of ZAP-70 + cells, while memory and follicular mantle B cells, which contain low numbers of activated B cells, expressed relatively little ZAP-70. A cell fraction of IgD +, CD38 + B cells, which are comprised of many in vivo activated B cells, exhibited the highest levels of ZAP-70. Density gradient fractionation of tonsil B cells confirmed that ZAP-70 was not expressed by resting B cells, but was expressed by buoyant, in vivo activated B cells. In these B cells, the expression of ZAP-70 correlated with that of CD38 and not with that of CD5, a hallmark of B-CLL cells. B-CLL cells are activated cells and their ZAP-70 expression reflects a normal property of activated B cells populations rather than a neoplastic aberration.

Original languageEnglish
Pages (from-to)558-569
Number of pages12
JournalEuropean Journal of Immunology
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 2006

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B-Lymphocytes
B-Cell Chronic Lymphocytic Leukemia
Palatine Tonsil
T-Lymphocytes
Immunoglobulin D
Germinal Center
Plasma Cells
Natural Killer Cells
Protein-Tyrosine Kinases
Complementary DNA

Keywords

  • Activated B cells
  • B cells subsets
  • B-CLL
  • Human B lymphocytes
  • ZAP-70

ASJC Scopus subject areas

  • Immunology

Cite this

B lymphocytes in humans express ZAP-70 when activated in vivo. / Cutrona, Giovanna; Colombo, Monica; Matis, Serena; Reverberi, Daniele; Dono, Mariella; Tarantino, Vicenzo; Chiorazzi, Nicholas; Ferrarini, Manlio.

In: European Journal of Immunology, Vol. 36, No. 3, 03.2006, p. 558-569.

Research output: Contribution to journalArticle

Cutrona, G, Colombo, M, Matis, S, Reverberi, D, Dono, M, Tarantino, V, Chiorazzi, N & Ferrarini, M 2006, 'B lymphocytes in humans express ZAP-70 when activated in vivo', European Journal of Immunology, vol. 36, no. 3, pp. 558-569. https://doi.org/10.1002/eji.200526355
Cutrona, Giovanna ; Colombo, Monica ; Matis, Serena ; Reverberi, Daniele ; Dono, Mariella ; Tarantino, Vicenzo ; Chiorazzi, Nicholas ; Ferrarini, Manlio. / B lymphocytes in humans express ZAP-70 when activated in vivo. In: European Journal of Immunology. 2006 ; Vol. 36, No. 3. pp. 558-569.
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