Abstract
ZAP-70 is a protein tyrosine kinase initially found in T and NK cells. Recently, this important signaling element was detected in leukemic B cells from a subgroup of patients with B cell chronic lymphocytic leukemia (B-CLL). In this study, ZAP-70 was detected in normal B cells from human tonsils, but not from peripheral blood. The cDNA sequence of B cell ZAP-70 was the same as that in T cells. Germinal center B cells and plasma cells had a substantial proportion of ZAP-70 + cells, while memory and follicular mantle B cells, which contain low numbers of activated B cells, expressed relatively little ZAP-70. A cell fraction of IgD +, CD38 + B cells, which are comprised of many in vivo activated B cells, exhibited the highest levels of ZAP-70. Density gradient fractionation of tonsil B cells confirmed that ZAP-70 was not expressed by resting B cells, but was expressed by buoyant, in vivo activated B cells. In these B cells, the expression of ZAP-70 correlated with that of CD38 and not with that of CD5, a hallmark of B-CLL cells. B-CLL cells are activated cells and their ZAP-70 expression reflects a normal property of activated B cells populations rather than a neoplastic aberration.
| Original language | English |
|---|---|
| Pages (from-to) | 558-569 |
| Number of pages | 12 |
| Journal | European Journal of Immunology |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2006 |
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Keywords
- Activated B cells
- B cells subsets
- B-CLL
- Human B lymphocytes
- ZAP-70
ASJC Scopus subject areas
- Immunology
Cite this
B lymphocytes in humans express ZAP-70 when activated in vivo. / Cutrona, Giovanna; Colombo, Monica; Matis, Serena; Reverberi, Daniele; Dono, Mariella; Tarantino, Vicenzo; Chiorazzi, Nicholas; Ferrarini, Manlio.
In: European Journal of Immunology, Vol. 36, No. 3, 03.2006, p. 558-569.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - B lymphocytes in humans express ZAP-70 when activated in vivo
AU - Cutrona, Giovanna
AU - Colombo, Monica
AU - Matis, Serena
AU - Reverberi, Daniele
AU - Dono, Mariella
AU - Tarantino, Vicenzo
AU - Chiorazzi, Nicholas
AU - Ferrarini, Manlio
PY - 2006/3
Y1 - 2006/3
N2 - ZAP-70 is a protein tyrosine kinase initially found in T and NK cells. Recently, this important signaling element was detected in leukemic B cells from a subgroup of patients with B cell chronic lymphocytic leukemia (B-CLL). In this study, ZAP-70 was detected in normal B cells from human tonsils, but not from peripheral blood. The cDNA sequence of B cell ZAP-70 was the same as that in T cells. Germinal center B cells and plasma cells had a substantial proportion of ZAP-70 + cells, while memory and follicular mantle B cells, which contain low numbers of activated B cells, expressed relatively little ZAP-70. A cell fraction of IgD +, CD38 + B cells, which are comprised of many in vivo activated B cells, exhibited the highest levels of ZAP-70. Density gradient fractionation of tonsil B cells confirmed that ZAP-70 was not expressed by resting B cells, but was expressed by buoyant, in vivo activated B cells. In these B cells, the expression of ZAP-70 correlated with that of CD38 and not with that of CD5, a hallmark of B-CLL cells. B-CLL cells are activated cells and their ZAP-70 expression reflects a normal property of activated B cells populations rather than a neoplastic aberration.
AB - ZAP-70 is a protein tyrosine kinase initially found in T and NK cells. Recently, this important signaling element was detected in leukemic B cells from a subgroup of patients with B cell chronic lymphocytic leukemia (B-CLL). In this study, ZAP-70 was detected in normal B cells from human tonsils, but not from peripheral blood. The cDNA sequence of B cell ZAP-70 was the same as that in T cells. Germinal center B cells and plasma cells had a substantial proportion of ZAP-70 + cells, while memory and follicular mantle B cells, which contain low numbers of activated B cells, expressed relatively little ZAP-70. A cell fraction of IgD +, CD38 + B cells, which are comprised of many in vivo activated B cells, exhibited the highest levels of ZAP-70. Density gradient fractionation of tonsil B cells confirmed that ZAP-70 was not expressed by resting B cells, but was expressed by buoyant, in vivo activated B cells. In these B cells, the expression of ZAP-70 correlated with that of CD38 and not with that of CD5, a hallmark of B-CLL cells. B-CLL cells are activated cells and their ZAP-70 expression reflects a normal property of activated B cells populations rather than a neoplastic aberration.
KW - Activated B cells
KW - B cells subsets
KW - B-CLL
KW - Human B lymphocytes
KW - ZAP-70
UR - http://www.scopus.com/inward/record.url?scp=33644953267&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644953267&partnerID=8YFLogxK
U2 - 10.1002/eji.200526355
DO - 10.1002/eji.200526355
M3 - Article
C2 - 16482508
AN - SCOPUS:33644953267
VL - 36
SP - 558
EP - 569
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 3
ER -