B lymphocytes inhibit human osteoclastogenesis by secretion of TGFβ

M. Neale Weitzmann, Simone Cenci, Jeff Haug, Chris Brown, John DiPersio, Roberto Pacifici

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The role of B lymphocytes in osteoclast (OC) formation is controversial, because both stimulatory and inhibitory effects of B-lineage cells on osteoclastogenesis and life span have been reported. In this study, we have investigated the effects of mature B cells on human osteoclastogenesis using cultures of peripheral blood stem cells (PBSC), a system that generates functional OCs in the absence of stromal cells. We report that B cells inhibit the formation of OCs and shorten the life span of mature OCs by secreting transforming growth factor β (TGFβ), a factor that induces apoptosis in these cells. The antiosteoclastogenic effects of B cells are abolished by addition of anti-TGFβ antibody to osteoclast cultures and mimicked by treatment of B cell-deprived PBSC cultures with recombinant TGFβ, thus confirming TGFβ as the B cell produced antiosteoclastogenic activity. Thus, the ability of B cells to downregulate osteoclastogenesis by secretion of the apoptotic cytokine TGFβ provides new insights into the ability of immune cells to regulate OC formation under basal and inflammatory conditions. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalJournal of Cellular Biochemistry
Volume78
Issue number2
DOIs
Publication statusPublished - 2000

Fingerprint

Lymphocytes
Transforming Growth Factors
Osteogenesis
B-Lymphocytes
Cells
Cell culture
Osteoclasts
Stem cells
Blood
Stromal Cells
Apoptosis
Cytokines
Down-Regulation
Cell Culture Techniques
Antibodies

Keywords

  • Cytokines
  • Human osteoclastogenesis
  • Lymphocytes
  • TGFβ

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

B lymphocytes inhibit human osteoclastogenesis by secretion of TGFβ. / Weitzmann, M. Neale; Cenci, Simone; Haug, Jeff; Brown, Chris; DiPersio, John; Pacifici, Roberto.

In: Journal of Cellular Biochemistry, Vol. 78, No. 2, 2000, p. 318-324.

Research output: Contribution to journalArticle

Weitzmann, M. Neale ; Cenci, Simone ; Haug, Jeff ; Brown, Chris ; DiPersio, John ; Pacifici, Roberto. / B lymphocytes inhibit human osteoclastogenesis by secretion of TGFβ. In: Journal of Cellular Biochemistry. 2000 ; Vol. 78, No. 2. pp. 318-324.
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