B-myb antisense oligonucleotides inhibit proliferation of human hematopoietic cell lines

M. Arsura, M. Introna, F. Passerini, A. Mantovani, J. Golay

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The B-myb gene is highly homologous to the c-myb protooncogene in several domains and also shares some of the functions of c-myb in that it can act as a transcriptional activator. In addition, the expression of both the B-myb and c-myb genes correlates with proliferation of normal hematopoietic cells. We investigated more directly the role of B-myb in proliferation of hematopoietic cell lines using B-myb-specific antisense oligonucleotides. We showed that several anti-B-myb oligonucleotides, complementary to distinct regions of the gene, inhibit significantly and in a dose-dependent manner the proliferation of all myeloid or lymphoid cell lines tested. This block in proliferation was not accompanied by detectable differentiation of U937 or HL60 cells to macrophages or granulocytes either spontaneously or after exposure to chemical agents. These data suggest that the B-myb gene, like c-myb, is necessary for hematopoietic cell proliferation.

Original languageEnglish
Pages (from-to)2708-2716
Number of pages9
JournalBlood
Volume79
Issue number10
Publication statusPublished - May 15 1992

Fingerprint

myb Genes
Antisense Oligonucleotides
Genes
Cells
Cell Line
U937 Cells
Macrophages
HL-60 Cells
Cell proliferation
Myeloid Cells
Granulocytes
Oligonucleotides
Cell Proliferation
Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Arsura, M., Introna, M., Passerini, F., Mantovani, A., & Golay, J. (1992). B-myb antisense oligonucleotides inhibit proliferation of human hematopoietic cell lines. Blood, 79(10), 2708-2716.

B-myb antisense oligonucleotides inhibit proliferation of human hematopoietic cell lines. / Arsura, M.; Introna, M.; Passerini, F.; Mantovani, A.; Golay, J.

In: Blood, Vol. 79, No. 10, 15.05.1992, p. 2708-2716.

Research output: Contribution to journalArticle

Arsura, M, Introna, M, Passerini, F, Mantovani, A & Golay, J 1992, 'B-myb antisense oligonucleotides inhibit proliferation of human hematopoietic cell lines', Blood, vol. 79, no. 10, pp. 2708-2716.
Arsura M, Introna M, Passerini F, Mantovani A, Golay J. B-myb antisense oligonucleotides inhibit proliferation of human hematopoietic cell lines. Blood. 1992 May 15;79(10):2708-2716.
Arsura, M. ; Introna, M. ; Passerini, F. ; Mantovani, A. ; Golay, J. / B-myb antisense oligonucleotides inhibit proliferation of human hematopoietic cell lines. In: Blood. 1992 ; Vol. 79, No. 10. pp. 2708-2716.
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