Abstract
The present study was undertaken with the aim of defining the differences, if any, in bacteremic infectability of some currently available arterial grafts. The following prostheses were investigated: two kinds of expanded polytetrafluoroethylene (PTFE); Dacron knitted double velour; Dacron knitted pretreated with gelatin. Forty beagle dogs were used. In each animal the infrarenal aorta was resected and replaced by means of Φ.6 mm segment of: PTFE type I 10 dogs; PTFE type II 10 dogs; Dacron knitted double velour 10 dogs; Dacron knitted pretreated with gelatin 10 dogs. At the end of the operation, each animal was administered IV with 100 mL of normal saline containing a suspension of Staphylococcus aureus coagulase-positive phage type 5504. The bacterial load was 105 in 5 animals of each group, 107 in the remaining 5. The graft was retrieved after forty-five days and studied in the microbiology laboratory (study of graft homogenate) and in the pathology laboratory (study by conventional microscopy of graft healing). When culture yielded a strain of Staph. aureus, this was retyped for phage 5504 specificity. All grafts were patent at the end of the experiment: partial thrombosis was, however, observed, coupled with a frank perigraft inflammation in: 3 PTFE type 1 (2 Staph.105, 1 Staph.107); 1 PTFE type 2 (Staph. 105); 1 Dacron knitted double velour (Staph. 107). Baterial colonies within the graft tissue were evident at microscopy in 4 PTFE grafts and in 1 gelatin-pretreated graft. Culture was positive and specific in all the gelatin-treated Dacron grafts and in 60% of the nonpretreated Dacron and PTFE grafts. An optimal result (good healing, no inflammation, no infection) was observed only in 3 PTFE and in 2 'conventional' Dacron grafts. The possibility is suggested that bacteria may be harbored in graft tissue without any clinically evident effect; moreover, some late graft infection could be attributable to an early bacteremic contamination.
| Original language | English |
|---|---|
| Pages (from-to) | 89-99 |
| Number of pages | 11 |
| Journal | Vascular Surgery |
| Volume | 25 |
| Issue number | 2 |
| Publication status | Published - 1991 |
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ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
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Bacteremic infectability of vascular grafts : An experimental study. / Cavallaro, A.; Sciacca, V.; Cisternino, S.; Di Marzo, L.; Mingoli, A.; Farina, C.; Gallo, P.; Bernucci, P.; Bordi, E.; De Mori, P.; Orefice, G.
In: Vascular Surgery, Vol. 25, No. 2, 1991, p. 89-99.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Bacteremic infectability of vascular grafts
T2 - An experimental study
AU - Cavallaro, A.
AU - Sciacca, V.
AU - Cisternino, S.
AU - Di Marzo, L.
AU - Mingoli, A.
AU - Farina, C.
AU - Gallo, P.
AU - Bernucci, P.
AU - Bordi, E.
AU - De Mori, P.
AU - Orefice, G.
PY - 1991
Y1 - 1991
N2 - The present study was undertaken with the aim of defining the differences, if any, in bacteremic infectability of some currently available arterial grafts. The following prostheses were investigated: two kinds of expanded polytetrafluoroethylene (PTFE); Dacron knitted double velour; Dacron knitted pretreated with gelatin. Forty beagle dogs were used. In each animal the infrarenal aorta was resected and replaced by means of Φ.6 mm segment of: PTFE type I 10 dogs; PTFE type II 10 dogs; Dacron knitted double velour 10 dogs; Dacron knitted pretreated with gelatin 10 dogs. At the end of the operation, each animal was administered IV with 100 mL of normal saline containing a suspension of Staphylococcus aureus coagulase-positive phage type 5504. The bacterial load was 105 in 5 animals of each group, 107 in the remaining 5. The graft was retrieved after forty-five days and studied in the microbiology laboratory (study of graft homogenate) and in the pathology laboratory (study by conventional microscopy of graft healing). When culture yielded a strain of Staph. aureus, this was retyped for phage 5504 specificity. All grafts were patent at the end of the experiment: partial thrombosis was, however, observed, coupled with a frank perigraft inflammation in: 3 PTFE type 1 (2 Staph.105, 1 Staph.107); 1 PTFE type 2 (Staph. 105); 1 Dacron knitted double velour (Staph. 107). Baterial colonies within the graft tissue were evident at microscopy in 4 PTFE grafts and in 1 gelatin-pretreated graft. Culture was positive and specific in all the gelatin-treated Dacron grafts and in 60% of the nonpretreated Dacron and PTFE grafts. An optimal result (good healing, no inflammation, no infection) was observed only in 3 PTFE and in 2 'conventional' Dacron grafts. The possibility is suggested that bacteria may be harbored in graft tissue without any clinically evident effect; moreover, some late graft infection could be attributable to an early bacteremic contamination.
AB - The present study was undertaken with the aim of defining the differences, if any, in bacteremic infectability of some currently available arterial grafts. The following prostheses were investigated: two kinds of expanded polytetrafluoroethylene (PTFE); Dacron knitted double velour; Dacron knitted pretreated with gelatin. Forty beagle dogs were used. In each animal the infrarenal aorta was resected and replaced by means of Φ.6 mm segment of: PTFE type I 10 dogs; PTFE type II 10 dogs; Dacron knitted double velour 10 dogs; Dacron knitted pretreated with gelatin 10 dogs. At the end of the operation, each animal was administered IV with 100 mL of normal saline containing a suspension of Staphylococcus aureus coagulase-positive phage type 5504. The bacterial load was 105 in 5 animals of each group, 107 in the remaining 5. The graft was retrieved after forty-five days and studied in the microbiology laboratory (study of graft homogenate) and in the pathology laboratory (study by conventional microscopy of graft healing). When culture yielded a strain of Staph. aureus, this was retyped for phage 5504 specificity. All grafts were patent at the end of the experiment: partial thrombosis was, however, observed, coupled with a frank perigraft inflammation in: 3 PTFE type 1 (2 Staph.105, 1 Staph.107); 1 PTFE type 2 (Staph. 105); 1 Dacron knitted double velour (Staph. 107). Baterial colonies within the graft tissue were evident at microscopy in 4 PTFE grafts and in 1 gelatin-pretreated graft. Culture was positive and specific in all the gelatin-treated Dacron grafts and in 60% of the nonpretreated Dacron and PTFE grafts. An optimal result (good healing, no inflammation, no infection) was observed only in 3 PTFE and in 2 'conventional' Dacron grafts. The possibility is suggested that bacteria may be harbored in graft tissue without any clinically evident effect; moreover, some late graft infection could be attributable to an early bacteremic contamination.
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M3 - Article
AN - SCOPUS:0025730027
VL - 25
SP - 89
EP - 99
JO - Vascular Surgery
JF - Vascular Surgery
SN - 0042-2835
IS - 2
ER -