Bacteria-induced neo-biosynthesis, stabilization, and surface expression of functional class I molecules in mouse dendritic cells

Maria Rescigno, Stefania Citterio, Clotilde Thèry, Michael Rittig, Donata Medaglini, Gianni Pozzi, Sebastian Amigorena, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we show that bacteria induce de novo synthesis of both major histocompatability complex (MHC) class I and H molecules in a mouse dendritic cell culture system. The neo-biosynthesis of MHC class I molecules is delayed as compared with that of MHC class II. Furthermore, bacteria stabilize MHC class I molecules by a 3-fold increase of their half-life. This has important consequences for the capacity of dendritic cells to present bacterial antigens in the draining lymph nodes. In addition, a model antigen, ovalbumin, expressed on the surface of recombinant Streptococcus gordonii is processed and presented on MHC class I molecules. This presentation is 106 times more efficient than that of soluble OVA protein. This exogenous pathway of MHC class I presentation is transporter associated with antigen processing (TAP)-dependent, indicating that there is a transport from phagolysosome to cytosol in dendritic cells. Thus, bacteria are shown to be a potentially useful mean for the correct delivery of exogenous antigens to be presented efficiently on MHC class I molecules.

Original languageEnglish
Pages (from-to)5229-5234
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number9
DOIs
Publication statusPublished - Apr 28 1998

ASJC Scopus subject areas

  • Genetics
  • General

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