TY - JOUR
T1 - Bacterial artificial chromosome transgenesis through pronuclear injection of fertilized mouse oocytes.
AU - Vintersten, Kristina
AU - Testa, Giuseppe
AU - Naumann, Ronald
AU - Anastassiadis, Konstantinos
AU - Stewart, A. Francis
PY - 2008
Y1 - 2008
N2 - In the mouse, conventional transgenes often produced unpredictable results mainly because they were too small to recapitulate a natural gene context. Bacterial artificial chromosomes (BACs) are large enough to encompass the natural context of most mammalian genes and consequently deliver more reliable recapitulations of their endogenous counterparts. Furthermore, recombineering methods now make it easy to engineer precise changes in a BAC transgene. Consequently, BACs have become the preferred vehicle for mouse transgenesis. Here, we detail methods for BAC transgenesis through pronuclear injection of fertilized oocytes.
AB - In the mouse, conventional transgenes often produced unpredictable results mainly because they were too small to recapitulate a natural gene context. Bacterial artificial chromosomes (BACs) are large enough to encompass the natural context of most mammalian genes and consequently deliver more reliable recapitulations of their endogenous counterparts. Furthermore, recombineering methods now make it easy to engineer precise changes in a BAC transgene. Consequently, BACs have become the preferred vehicle for mouse transgenesis. Here, we detail methods for BAC transgenesis through pronuclear injection of fertilized oocytes.
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M3 - Article
C2 - 18370149
AN - SCOPUS:45549102726
VL - 415
SP - 83
EP - 100
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -