Bacterial lipopolysaccharide-induced hyporeactivity in perfused rat resistance vessels

Modulating effects of dexamethasone

M. Serio, M. A. Potenza, M. Montagnani, G. Mansi, R. Rinaldi, S. Pece, D. Fumarola, E. Jirillo, D. Mitolo-Chieppa

Research output: Contribution to journalArticle

Abstract

The present study was carried out on mesenteric vascular bed from LPS-injected rats in order to investigate possible mechanisms underlying hyporesponsiveness in resistance blood vessels in the course of septic shock syndrome. The involvement of L-arginine (L-Arg)/nitric oxide (NO) pathway was evaluated by administration of L-Arg, which produced a decrease in perfusion pressure in LPS-treated rats, whereas it was ineffective in control rats. Of note, dexamethasone (DEX) pretreatment in endotoxaemic rats significantly reduced the vasorelaxation by L-Arg; however, this non selective inhibitor of inducible-NOS expression was not able to prevent noradrenaline (NA) hyporeactivity. Furthermore, in order to evaluate whether hyporesponsiveness could be due to an altered contraction mechanism, the effect of endothelin (ET)-1 was tested. This peptide was able to markedly enhance the contractile response to noradrenaline in LPS-treated rats. Collectively, our findings suggest that vascular hyporesponsiveness during septic shock can only be partially explained by activation of the L-Arg/NO pathway. Other mechanisms, probably related to smooth muscle cell contractility, may be involved.

Original languageEnglish
Pages (from-to)491-496
Number of pages6
JournalInnate Immunity
Volume3
Issue number6
DOIs
Publication statusPublished - 1996

Fingerprint

Dexamethasone
Lipopolysaccharides
Blood Vessels
Septic Shock
Norepinephrine
Endothelin-1
Vasodilation
Smooth Muscle Myocytes
Arginine
Nitric Oxide
Perfusion
Pressure
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Immunology
  • Microbiology
  • Infectious Diseases

Cite this

Bacterial lipopolysaccharide-induced hyporeactivity in perfused rat resistance vessels : Modulating effects of dexamethasone. / Serio, M.; Potenza, M. A.; Montagnani, M.; Mansi, G.; Rinaldi, R.; Pece, S.; Fumarola, D.; Jirillo, E.; Mitolo-Chieppa, D.

In: Innate Immunity, Vol. 3, No. 6, 1996, p. 491-496.

Research output: Contribution to journalArticle

Serio, M, Potenza, MA, Montagnani, M, Mansi, G, Rinaldi, R, Pece, S, Fumarola, D, Jirillo, E & Mitolo-Chieppa, D 1996, 'Bacterial lipopolysaccharide-induced hyporeactivity in perfused rat resistance vessels: Modulating effects of dexamethasone', Innate Immunity, vol. 3, no. 6, pp. 491-496. https://doi.org/10.1177/096805199600300607
Serio, M. ; Potenza, M. A. ; Montagnani, M. ; Mansi, G. ; Rinaldi, R. ; Pece, S. ; Fumarola, D. ; Jirillo, E. ; Mitolo-Chieppa, D. / Bacterial lipopolysaccharide-induced hyporeactivity in perfused rat resistance vessels : Modulating effects of dexamethasone. In: Innate Immunity. 1996 ; Vol. 3, No. 6. pp. 491-496.
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