Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model

R. Baccala, B. A E Vandekerckhove, D. Jones, D. H. Kono, M. G. Roncarolo, A. N. Theofilopoulos

Research output: Contribution to journalArticle

Abstract

The ability to analyze T cell receptor (TCR) thymic repertoire shaping in humans by self and foreign ligands is hampered by the lack of suitable models. We recently documented that the mouse severe combined immunodeficiency (SCID)-human fetal liver/thymus model recapitulates the TCR Vβ gene repertoire of human thymocytes. Here, we show that an exogenous superantigen, staphylococcal enterotoxin B, administered to such mice induces clonal deletions in both CD4+8- and CD8+4- cells involving the same human Vβ clones that are selected in vitro by this toxin. This model, therefore, may allow comprehensive studies into the effects of microbial and other agents on human T cell thymic selection processes in a biologically relevant setting.

Original languageEnglish
Pages (from-to)1481-1485
Number of pages5
JournalJournal of Experimental Medicine
Volume177
Issue number5
Publication statusPublished - May 1 1993

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Severe Combined Immunodeficiency
Superantigens
Thymus Gland
T-Lymphocytes
Liver
Clonal Deletion
T-Cell Receptor Genes
Thymocytes
T-Cell Antigen Receptor
Clone Cells
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Baccala, R., Vandekerckhove, B. A. E., Jones, D., Kono, D. H., Roncarolo, M. G., & Theofilopoulos, A. N. (1993). Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model. Journal of Experimental Medicine, 177(5), 1481-1485.

Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model. / Baccala, R.; Vandekerckhove, B. A E; Jones, D.; Kono, D. H.; Roncarolo, M. G.; Theofilopoulos, A. N.

In: Journal of Experimental Medicine, Vol. 177, No. 5, 01.05.1993, p. 1481-1485.

Research output: Contribution to journalArticle

Baccala, R, Vandekerckhove, BAE, Jones, D, Kono, DH, Roncarolo, MG & Theofilopoulos, AN 1993, 'Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model', Journal of Experimental Medicine, vol. 177, no. 5, pp. 1481-1485.
Baccala R, Vandekerckhove BAE, Jones D, Kono DH, Roncarolo MG, Theofilopoulos AN. Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model. Journal of Experimental Medicine. 1993 May 1;177(5):1481-1485.
Baccala, R. ; Vandekerckhove, B. A E ; Jones, D. ; Kono, D. H. ; Roncarolo, M. G. ; Theofilopoulos, A. N. / Bacterial superantigens mediate T cell deletions in the mouse severe combined immunodeficiency-human liver/thymus model. In: Journal of Experimental Medicine. 1993 ; Vol. 177, No. 5. pp. 1481-1485.
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