Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells

Gianfranco Donelli, Alessia Fabbri, Carla Fiorentini

Research output: Contribution to journalArticle

Abstract

Certain strains of the anaerobic bacterium Bacteroides fragilis are known to produce an enterotoxin of about 20 kDa which is able to induce a fluid response in ligated intestinal loops and a cytotoxic response in HT-29 cells. It presents protease activity, belonging to a family of metalloproteases termed metzincins. In order to investigate the mode of action of the enterotoxin in cultured cells, we performed a study with HT-29 cells, using both fluorescence and electron microscopy. Treated cells underwent morphological changes, mainly consisting of the retraction of the cell body and the formation of numerous blebs on the cell surface. The microfilament system was reorganized, the F-actin being condensed as a ring at the cell periphery, whereas other cell organelles appeared to be unaffected. All these changes, clearly visible after 3 h of exposure to the toxin, were reversed within 24 h of treatment. By inhibiting the protease activity of the toxin with specific metal chelators, the cytoskeletal effects were also prevented. Thus, B. fragilis enterotoxin appears to act on cells by reversibly modifying the actin cytoskeleton, an effect probably dependent on its proteolytic activity.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalInfection and Immunity
Volume64
Issue number1
Publication statusPublished - 1996

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HT29 Cells
Blister
Enterotoxins
Actin Cytoskeleton
Peptide Hydrolases
Bacteroides fragilis
Anaerobic Bacteria
Metalloproteases
Chelating Agents
Fluorescence Microscopy
Organelles
Actins
Cultured Cells
Electron Microscopy
Metals
fragilysin

ASJC Scopus subject areas

  • Immunology

Cite this

Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells. / Donelli, Gianfranco; Fabbri, Alessia; Fiorentini, Carla.

In: Infection and Immunity, Vol. 64, No. 1, 1996, p. 113-119.

Research output: Contribution to journalArticle

Donelli, Gianfranco ; Fabbri, Alessia ; Fiorentini, Carla. / Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells. In: Infection and Immunity. 1996 ; Vol. 64, No. 1. pp. 113-119.
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