Abstract
Background: BAMBI is a type I TGFβ receptor antagonist, whose in vivo function remains unclear, as BAMBI-/- mice lack an obvious phenotype. Methodology/Principal Findings: Identifying BAMBI's functions requires identification of cell-specific expression of BAMBI. By immunohistology we found BAMBI expression restricted to endothelial cells and by electron microscopy BAMBI-/- mice showed prominent and swollen endothelial cells in myocardial and glomerular capillaries. In endothelial cells over-expression of BAMBI reduced, whereas knock-down enhanced capillary growth and migration in response to TGFβ. In vivo angiogenesis was enhanced in matrigel implants and in glomerular hypertrophy after unilateral nephrectomy in BAMBI-/- compared to BAMBI+/+ mice consistent with an endothelial phenotype for BAMBI-/- mice. BAMBI's mechanism of action in endothelial cells was examined by canonical and alternative TGFβ signaling in HUVEC with over-expression or knock-down of BAMBI. BAMBI knockdown enhanced basal and TGFβ stimulated SMAD1/5 and ERK1/2 phosphorylation, while over-expression prevented both. Conclusions/Significance: Thus we provide a first description of a vascular phenotype for BAMBI-/- mice, and provide in vitro and in vivo evidence that BAMBI contributes to endothelial and vascular homeostasis. Further, we demonstrate that in endothelial cells BAMBI interferes with alternative TGFβ signaling, most likely through the ALK 1 receptor, which may explain the phenotype observed in BAMBI-/- mice. This newly described role for BAMBI in regulating endothelial function has potential implications for understanding and treating vascular disease and tumor neo-angiogenesis.
| Original language | English |
|---|---|
| Article number | e39406 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 25 2012 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling. / Guillot, Nicolas; Kollins, Dmitrij; Gilbert, Victoria; Xavier, Sandhya; Chen, Jun; Gentle, Madeleine; Reddy, Anand; Bottinger, Erwin; Jiang, Rulang; Rastaldi, Maria Pia; Corbelli, Alessandro; Schlondorff, Detlef.
In: PLoS One, Vol. 7, No. 6, e39406, 25.06.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling
AU - Guillot, Nicolas
AU - Kollins, Dmitrij
AU - Gilbert, Victoria
AU - Xavier, Sandhya
AU - Chen, Jun
AU - Gentle, Madeleine
AU - Reddy, Anand
AU - Bottinger, Erwin
AU - Jiang, Rulang
AU - Rastaldi, Maria Pia
AU - Corbelli, Alessandro
AU - Schlondorff, Detlef
PY - 2012/6/25
Y1 - 2012/6/25
N2 - Background: BAMBI is a type I TGFβ receptor antagonist, whose in vivo function remains unclear, as BAMBI-/- mice lack an obvious phenotype. Methodology/Principal Findings: Identifying BAMBI's functions requires identification of cell-specific expression of BAMBI. By immunohistology we found BAMBI expression restricted to endothelial cells and by electron microscopy BAMBI-/- mice showed prominent and swollen endothelial cells in myocardial and glomerular capillaries. In endothelial cells over-expression of BAMBI reduced, whereas knock-down enhanced capillary growth and migration in response to TGFβ. In vivo angiogenesis was enhanced in matrigel implants and in glomerular hypertrophy after unilateral nephrectomy in BAMBI-/- compared to BAMBI+/+ mice consistent with an endothelial phenotype for BAMBI-/- mice. BAMBI's mechanism of action in endothelial cells was examined by canonical and alternative TGFβ signaling in HUVEC with over-expression or knock-down of BAMBI. BAMBI knockdown enhanced basal and TGFβ stimulated SMAD1/5 and ERK1/2 phosphorylation, while over-expression prevented both. Conclusions/Significance: Thus we provide a first description of a vascular phenotype for BAMBI-/- mice, and provide in vitro and in vivo evidence that BAMBI contributes to endothelial and vascular homeostasis. Further, we demonstrate that in endothelial cells BAMBI interferes with alternative TGFβ signaling, most likely through the ALK 1 receptor, which may explain the phenotype observed in BAMBI-/- mice. This newly described role for BAMBI in regulating endothelial function has potential implications for understanding and treating vascular disease and tumor neo-angiogenesis.
AB - Background: BAMBI is a type I TGFβ receptor antagonist, whose in vivo function remains unclear, as BAMBI-/- mice lack an obvious phenotype. Methodology/Principal Findings: Identifying BAMBI's functions requires identification of cell-specific expression of BAMBI. By immunohistology we found BAMBI expression restricted to endothelial cells and by electron microscopy BAMBI-/- mice showed prominent and swollen endothelial cells in myocardial and glomerular capillaries. In endothelial cells over-expression of BAMBI reduced, whereas knock-down enhanced capillary growth and migration in response to TGFβ. In vivo angiogenesis was enhanced in matrigel implants and in glomerular hypertrophy after unilateral nephrectomy in BAMBI-/- compared to BAMBI+/+ mice consistent with an endothelial phenotype for BAMBI-/- mice. BAMBI's mechanism of action in endothelial cells was examined by canonical and alternative TGFβ signaling in HUVEC with over-expression or knock-down of BAMBI. BAMBI knockdown enhanced basal and TGFβ stimulated SMAD1/5 and ERK1/2 phosphorylation, while over-expression prevented both. Conclusions/Significance: Thus we provide a first description of a vascular phenotype for BAMBI-/- mice, and provide in vitro and in vivo evidence that BAMBI contributes to endothelial and vascular homeostasis. Further, we demonstrate that in endothelial cells BAMBI interferes with alternative TGFβ signaling, most likely through the ALK 1 receptor, which may explain the phenotype observed in BAMBI-/- mice. This newly described role for BAMBI in regulating endothelial function has potential implications for understanding and treating vascular disease and tumor neo-angiogenesis.
UR - http://www.scopus.com/inward/record.url?scp=84862668276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862668276&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0039406
DO - 10.1371/journal.pone.0039406
M3 - Article
C2 - 22761782
AN - SCOPUS:84862668276
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 6
M1 - e39406
ER -