BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling

Nicolas Guillot, Dmitrij Kollins, Victoria Gilbert, Sandhya Xavier, Jun Chen, Madeleine Gentle, Anand Reddy, Erwin Bottinger, Rulang Jiang, Maria Pia Rastaldi, Alessandro Corbelli, Detlef Schlondorff

Research output: Contribution to journalArticle

Abstract

Background: BAMBI is a type I TGFβ receptor antagonist, whose in vivo function remains unclear, as BAMBI-/- mice lack an obvious phenotype. Methodology/Principal Findings: Identifying BAMBI's functions requires identification of cell-specific expression of BAMBI. By immunohistology we found BAMBI expression restricted to endothelial cells and by electron microscopy BAMBI-/- mice showed prominent and swollen endothelial cells in myocardial and glomerular capillaries. In endothelial cells over-expression of BAMBI reduced, whereas knock-down enhanced capillary growth and migration in response to TGFβ. In vivo angiogenesis was enhanced in matrigel implants and in glomerular hypertrophy after unilateral nephrectomy in BAMBI-/- compared to BAMBI+/+ mice consistent with an endothelial phenotype for BAMBI-/- mice. BAMBI's mechanism of action in endothelial cells was examined by canonical and alternative TGFβ signaling in HUVEC with over-expression or knock-down of BAMBI. BAMBI knockdown enhanced basal and TGFβ stimulated SMAD1/5 and ERK1/2 phosphorylation, while over-expression prevented both. Conclusions/Significance: Thus we provide a first description of a vascular phenotype for BAMBI-/- mice, and provide in vitro and in vivo evidence that BAMBI contributes to endothelial and vascular homeostasis. Further, we demonstrate that in endothelial cells BAMBI interferes with alternative TGFβ signaling, most likely through the ALK 1 receptor, which may explain the phenotype observed in BAMBI-/- mice. This newly described role for BAMBI in regulating endothelial function has potential implications for understanding and treating vascular disease and tumor neo-angiogenesis.

Original languageEnglish
Article numbere39406
JournalPLoS One
Volume7
Issue number6
DOIs
Publication statusPublished - Jun 25 2012

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Endothelial cells
angiogenesis
homeostasis
Homeostasis
endothelial cells
Modulation
Endothelial Cells
mice
Phenotype
phenotype
blood vessels
Blood Vessels
Phosphorylation
receptors
vascular diseases
Electron microscopy
Tumors
Nephrectomy
Vascular Diseases
hypertrophy

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Guillot, N., Kollins, D., Gilbert, V., Xavier, S., Chen, J., Gentle, M., ... Schlondorff, D. (2012). BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling. PLoS One, 7(6), [e39406]. https://doi.org/10.1371/journal.pone.0039406

BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling. / Guillot, Nicolas; Kollins, Dmitrij; Gilbert, Victoria; Xavier, Sandhya; Chen, Jun; Gentle, Madeleine; Reddy, Anand; Bottinger, Erwin; Jiang, Rulang; Rastaldi, Maria Pia; Corbelli, Alessandro; Schlondorff, Detlef.

In: PLoS One, Vol. 7, No. 6, e39406, 25.06.2012.

Research output: Contribution to journalArticle

Guillot, N, Kollins, D, Gilbert, V, Xavier, S, Chen, J, Gentle, M, Reddy, A, Bottinger, E, Jiang, R, Rastaldi, MP, Corbelli, A & Schlondorff, D 2012, 'BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling', PLoS One, vol. 7, no. 6, e39406. https://doi.org/10.1371/journal.pone.0039406
Guillot, Nicolas ; Kollins, Dmitrij ; Gilbert, Victoria ; Xavier, Sandhya ; Chen, Jun ; Gentle, Madeleine ; Reddy, Anand ; Bottinger, Erwin ; Jiang, Rulang ; Rastaldi, Maria Pia ; Corbelli, Alessandro ; Schlondorff, Detlef. / BAMBI regulates angiogenesis and endothelial homeostasis through modulation of alternative TGFβ signaling. In: PLoS One. 2012 ; Vol. 7, No. 6.
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AU - Bottinger, Erwin

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