Abstract
Encapsulation in a barium alginate membrane is a promising strategy to obtain a three dimensional culture of living cells: membrane properties are crucial for a realistic clinical application. A one-step encapsulation technique, recently developed for controlled release of boar semen, was employed to prepare barium alginate and protamine-alginate membranes: permeability to two model molecules (haemoglobin and glucose) was evaluated. Capsules were evaluated for technological properties and scanning electron microscopy was used to examine the external morphology of the capsules and the 3D distribution of the cells within the core. The results indicate that 3D arrangement and the cell shape are maintained, capsule dimensions and mechanical properties can be modulated, as well as their permeability to model molecules such as haemoglobin and glucose.
| Original language | English |
|---|---|
| Pages (from-to) | 167-172 |
| Number of pages | 6 |
| Journal | Journal of Drug Delivery Science and Technology |
| Volume | 14 |
| Issue number | 3 |
| Publication status | Published - May 2004 |
Fingerprint
Keywords
- 3D cell arrangement
- Alginate
- Cell encapsulation
- Membrane permeability
- SEM
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
Barium alginate capsules for 3D immobilisation of living cells : Morphology, membrane properties and permeability. / Vigo, D.; Torre, M. L.; Faustini, M.; Munari, E.; Russo, V.; Norberti, R.; Villani, S.; Asti, A.; Bini, P. P.; Conte, U.
In: Journal of Drug Delivery Science and Technology, Vol. 14, No. 3, 05.2004, p. 167-172.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Barium alginate capsules for 3D immobilisation of living cells
T2 - Morphology, membrane properties and permeability
AU - Vigo, D.
AU - Torre, M. L.
AU - Faustini, M.
AU - Munari, E.
AU - Russo, V.
AU - Norberti, R.
AU - Villani, S.
AU - Asti, A.
AU - Bini, P. P.
AU - Conte, U.
PY - 2004/5
Y1 - 2004/5
N2 - Encapsulation in a barium alginate membrane is a promising strategy to obtain a three dimensional culture of living cells: membrane properties are crucial for a realistic clinical application. A one-step encapsulation technique, recently developed for controlled release of boar semen, was employed to prepare barium alginate and protamine-alginate membranes: permeability to two model molecules (haemoglobin and glucose) was evaluated. Capsules were evaluated for technological properties and scanning electron microscopy was used to examine the external morphology of the capsules and the 3D distribution of the cells within the core. The results indicate that 3D arrangement and the cell shape are maintained, capsule dimensions and mechanical properties can be modulated, as well as their permeability to model molecules such as haemoglobin and glucose.
AB - Encapsulation in a barium alginate membrane is a promising strategy to obtain a three dimensional culture of living cells: membrane properties are crucial for a realistic clinical application. A one-step encapsulation technique, recently developed for controlled release of boar semen, was employed to prepare barium alginate and protamine-alginate membranes: permeability to two model molecules (haemoglobin and glucose) was evaluated. Capsules were evaluated for technological properties and scanning electron microscopy was used to examine the external morphology of the capsules and the 3D distribution of the cells within the core. The results indicate that 3D arrangement and the cell shape are maintained, capsule dimensions and mechanical properties can be modulated, as well as their permeability to model molecules such as haemoglobin and glucose.
KW - 3D cell arrangement
KW - Alginate
KW - Cell encapsulation
KW - Membrane permeability
KW - SEM
UR - http://www.scopus.com/inward/record.url?scp=2942715309&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942715309&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:2942715309
VL - 14
SP - 167
EP - 172
JO - Journal of Drug Delivery Science and Technology
JF - Journal of Drug Delivery Science and Technology
SN - 1773-2247
IS - 3
ER -