Baseline micronuclei frequency in children

Estimates from meta- and pooled analyses

Monica Neri, Marcello Ceppi, Lisbeth E. Knudsen, Domenico Franco Merlo, Roberto Barale, Riccardo Puntoni, Stefano Bonassi

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95% confidence interval (CI), 3.35-5.98] and 5.70 (95% CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.

Original languageEnglish
Pages (from-to)1226-1229
Number of pages4
JournalEnvironmental Health Perspectives
Volume113
Issue number9
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Meta-Analysis
Pediatrics
Lymphocytes
meta-analysis
Biomarkers
confidence interval
Assays
Blood
Availability
Databases
Confidence Intervals
Cytokinesis
Environmental Exposure
biomarker
blood
Newborn Infant
assay
analysis
Population
effect

Keywords

  • Biomarker
  • Children
  • Environmental exposure
  • Genetic damage
  • Meta-analysis
  • Micronucleus assay
  • Molecular epidemiology
  • Pooled analysis

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Baseline micronuclei frequency in children : Estimates from meta- and pooled analyses. / Neri, Monica; Ceppi, Marcello; Knudsen, Lisbeth E.; Merlo, Domenico Franco; Barale, Roberto; Puntoni, Riccardo; Bonassi, Stefano.

In: Environmental Health Perspectives, Vol. 113, No. 9, 09.2005, p. 1226-1229.

Research output: Contribution to journalArticle

Neri, Monica ; Ceppi, Marcello ; Knudsen, Lisbeth E. ; Merlo, Domenico Franco ; Barale, Roberto ; Puntoni, Riccardo ; Bonassi, Stefano. / Baseline micronuclei frequency in children : Estimates from meta- and pooled analyses. In: Environmental Health Perspectives. 2005 ; Vol. 113, No. 9. pp. 1226-1229.
@article{50f4c8ae0c994106a9d91d21604f6edd,
title = "Baseline micronuclei frequency in children: Estimates from meta- and pooled analyses",
abstract = "The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95{\%} confidence interval (CI), 3.35-5.98] and 5.70 (95{\%} CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.",
keywords = "Biomarker, Children, Environmental exposure, Genetic damage, Meta-analysis, Micronucleus assay, Molecular epidemiology, Pooled analysis",
author = "Monica Neri and Marcello Ceppi and Knudsen, {Lisbeth E.} and Merlo, {Domenico Franco} and Roberto Barale and Riccardo Puntoni and Stefano Bonassi",
year = "2005",
month = "9",
doi = "10.1289/ehp.7806",
language = "English",
volume = "113",
pages = "1226--1229",
journal = "Environmental Health Perspectives",
issn = "0091-6765",
publisher = "Public Health Services, US Dept of Health and Human Services",
number = "9",

}

TY - JOUR

T1 - Baseline micronuclei frequency in children

T2 - Estimates from meta- and pooled analyses

AU - Neri, Monica

AU - Ceppi, Marcello

AU - Knudsen, Lisbeth E.

AU - Merlo, Domenico Franco

AU - Barale, Roberto

AU - Puntoni, Riccardo

AU - Bonassi, Stefano

PY - 2005/9

Y1 - 2005/9

N2 - The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95% confidence interval (CI), 3.35-5.98] and 5.70 (95% CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.

AB - The number of studies evaluating the effect of environmental exposure to genotoxic agents in children has rapidly increased in the last few years. The frequency of micronuclei (MN) in peripheral blood lymphocytes determined with the cytokinesis block assay is among the most popular biomarkers used for this purpose, although large inter- and intralaboratory variability of this end point has been observed in population studies. The availability of reference measures is therefore necessary for laboratories to validate protocols and analytical procedures, and for molecular epidemiologists, as well, to estimate the statistical power of studies and to assess the quality of data. In this article, we provide estimates of the baseline frequency of MN in children, conducting a meta-analysis of MN frequency reported by field studies in children and a pooled analysis of individual data [available from published studies and from the Human Micronucleus International Collaborative Study (HUMN) database]. Thirteen articles were selected for meta-analysis, and individual data included in the pooled analysis were retrieved from the databases of 12 laboratories. Overall means of 4.48 [95% confidence interval (CI), 3.35-5.98] and 5.70 (95% CI, 4.29-7.56) MN per 1,000 binucleated cells were estimated by the meta- and pooled analysis, respectively. A clear effect of age was detected, even within the restricted range of pediatric age considered, with significantly lower frequency values in newborns. No influence of sex was found. The study showed the advantage of using data from large collaborative studies and suggested a synergistic use of meta- and pooled analysis.

KW - Biomarker

KW - Children

KW - Environmental exposure

KW - Genetic damage

KW - Meta-analysis

KW - Micronucleus assay

KW - Molecular epidemiology

KW - Pooled analysis

UR - http://www.scopus.com/inward/record.url?scp=25844435718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25844435718&partnerID=8YFLogxK

U2 - 10.1289/ehp.7806

DO - 10.1289/ehp.7806

M3 - Article

VL - 113

SP - 1226

EP - 1229

JO - Environmental Health Perspectives

JF - Environmental Health Perspectives

SN - 0091-6765

IS - 9

ER -