Basic amino acids and dimethylarginines targeted metabolomics discriminates primary hepatocarcinoma from hepatic colorectal metastases

Oceania D’Apolito, Daniela Garofalo, Monica Gelzo, Debora Paris, Dominique Melck, Rosa Calemma, Francesco Izzo, Giuseppe Palmieri, Giuseppe Castello, Andrea Motta, Gaetano Corso

Research output: Contribution to journalArticle

Abstract

Hepatocellular carcinoma (HCC) is a very aggressive neoplasia requiring early and accurate diagnosis to improve patient outcomes with timely treatment. The liver is also very frequently colonized by metastases, and the most frequent differential diagnosis is HCC against intrahepatic cholangiocarcinoma or metastatic adenocarcinoma. Metabolomics is a powerful tool for identification of altered biomarkers in cancer, and to evaluate the efficacy of drug treatments. Here we analyzed by HILIC-MS/MS methylated arginines, basic amino acids (Arg, Cit, Orn), and their ratios in the extracts of primary HCC tissues, liver metastases from colorectal carcinoma (MET), cirrhotic related hepatitis-C-virus (CIR), and non-cirrhotic normal liver (NT) adjacent tissues. We found high levels of Arg (p 

Original languageEnglish
Article number0
Pages (from-to)1026-1035
Number of pages10
JournalMetabolomics
Volume10
Issue number5
DOIs
Publication statusPublished - Oct 1 2014

Keywords

  • Arginine
  • Basic amino acids
  • Dimethylarginines
  • Hepatic tumorigenesis
  • Mass spectrometry
  • Metastases
  • Tissue metabolic profiling

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Fingerprint Dive into the research topics of 'Basic amino acids and dimethylarginines targeted metabolomics discriminates primary hepatocarcinoma from hepatic colorectal metastases'. Together they form a unique fingerprint.

  • Cite this

    D’Apolito, O., Garofalo, D., Gelzo, M., Paris, D., Melck, D., Calemma, R., Izzo, F., Palmieri, G., Castello, G., Motta, A., & Corso, G. (2014). Basic amino acids and dimethylarginines targeted metabolomics discriminates primary hepatocarcinoma from hepatic colorectal metastases. Metabolomics, 10(5), 1026-1035. [0]. https://doi.org/10.1007/s11306-014-0641-2