Basic and clinical immunology: Markers of cell death-activation in lymphocytes of vertically HIV-infected children naive to highly active antiretroviral therapy: The role of age

Alessandra Viganò, Marcello Pinti, Milena Nasi, Laura Moretti, Fiorella Balli, Cristina Mussini, Dorella Bricalli, Natascia Sala, Roberto Bugarini, Stefano Vella, Nicola Principi, Andrea Cossarizza

Research output: Contribution to journalArticle

Abstract

Background: Apoptosis plays a major role in depleting CD4 + lymphocytes during infection with HIV-1. Few data exist on its role during HIV infection of children. Sensitivity of peripheral blood lymphocytes (PBLs) to apoptotic stimuli and the importance of the patient's age remain unclear. Objectives: We sought to analyze the following: (1) markers of cell death-activation (CD95, CD45 isoforms, and CD28) in PBLs from vertically HIV-infected children of different ages before highly active antiretroviral therapy; (2) changes in other PBL populations; (3) PBL sensitivity to cell death and mitochondrial damages; and (4) role of age during progression of infection. Methods: Cell culture techniques and flow cytometry were used to analyze surface antigens, PBL susceptibility to apoptosis, or PBL susceptibility to change of mitochondrial membrane potential. Results: Donor age had a strong negative correlation with numbers of CD4 + and CD8 + T cells. Virgin T lymphocyte (CD45RA +, CD95 -) levels and those of CD95 + cells showed no correlation with the children's clinical status but did show a correlation with patient age. CD28 - T lymphocytes were markedly augmented in HIV-infected children but were unrelated to stage of infection or age. A relevant decrease in B lymphocytes and an increase in natural killer cells were also found. Finally, PBLs from HIV-positive children had a marked tendency to undergo apoptosis and mitochondrial damage. Conclusion: Changes in PBL phenotype, increased expression of CD95, and high sensitivity to apoptosis suggest that a precocious aging of the immune system occurs in HIV-infected children.

Original languageEnglish
Pages (from-to)439-445
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume108
Issue number3
DOIs
Publication statusPublished - 2001

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Keywords

  • Apoptosis
  • CD95
  • CD95L
  • HIV
  • Mitochondria
  • Pediatric AIDS

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Viganò, A., Pinti, M., Nasi, M., Moretti, L., Balli, F., Mussini, C., Bricalli, D., Sala, N., Bugarini, R., Vella, S., Principi, N., & Cossarizza, A. (2001). Basic and clinical immunology: Markers of cell death-activation in lymphocytes of vertically HIV-infected children naive to highly active antiretroviral therapy: The role of age. Journal of Allergy and Clinical Immunology, 108(3), 439-445. https://doi.org/10.1067/mai.2001.117791